Winkler Team

Group photo July 2017

Research Goals

Margit Winkler is fascinated by the power of natural biocatalysts for chemical synthesis. Many of the reactions that one may want to use for the preparation of organic molecules have not yet been developed, or have some limitations such as: the need for highly complicated and expensive catalysts, multiple steps or they simply suffer from low selectivity. The overall theme of Margit's research is to find new and alternative ways to catalyse a transformation by using enzymes. Sometimes, the goal is to determine the scope and limitations of an enzyme and possibly its engineering towards desired characteristics to overcome these limitations. Other times, it is the discovery and detailed study of enzymes that are not well known yet, in order to understand them better and to determine whether and how they could become applicable for synthesis. And finally, a particular product may be in the focus of the studies and the goal is then to find the best way to prepare it. Due to this broad portfolio of interests and needs, enzymes, substrates, products and methods that have been in the focus of the team over the years, it is essential to collaborate vividly and connect the knowledge of diverse fields. 

To top

Partners

To top

Selected Publications

2025
Jonathan Guyang Ling, Hannah G. Breuer, Holly Stolterfoht-Stock, Farah Diba Abu Bakar and Margit Winkler NADH Driven Enzymatic Carboxylic Acid Reduction Publikation in PURE anzeigen
Astrid Schiefer, Lukas Schober, Thomas Rohr, Margit Winkler and Florian Rudroff Co-enzyme-free, enzymatic synthesis of aldehydes from renewable resources with a new and highly efficient alkene cleaving dioxygenase Publikation in PURE anzeigen
Lukas Schober, Jacek Plewka, Kanokkan Sriwaiyaphram, Björn Bielec, Astrid Schiefer, Thanyaporn Wongnate, Katarzyna Magiera-Mularz, Florian Rudroff and Margit Winkler Structural and Functional Characteristics of Potent Dioxygenase from Moesziomyces aphidis Publikation in PURE anzeigen
Ana-Katarina Maric, Holly Stolterfoht-Stock, Margit Winkler, Martina Sudar and Zvjezdana Findrik Blažević Carboxylic acid reductase: a key for enzymatic aldehyde synthesis Publikation in PURE anzeigen
Pietro Caramia, Antonino Biundo, Silvia D'Eusebio, Isabella Pisano, Margit Winkler and Gennaro Agrimi Microbial cell factories for the sustainable valorization of Waste Cooking Oils into diols via enzymatic cascades Publikation in PURE anzeigen
Andrea Nigl, Veronica Delsoglio, Lucija Sovic, Marina Grgić, Lenny Malihan-Yap, Kamela K. Myrtollari, Jelena Spasic, Margit Winkler, Gustav Oberdorfer, Andreas Taden, Iva Anić and Robert Kourist Engineering of Transmembrane Alkane Monooxygenases to Improve a Key Reaction Step in the Synthesis of Polymer Precursor Tulipalin A Publikation in PURE anzeigen
2024
Simon Arhar, Thomas Rauter, Holly Stolterfoht, Vera Lambauer, Regina Kratzer, Margit Winkler, Marianna Karava, Robert Kourist and Anita Emmerstorfer-Augustin CO2-based production of phytase from highly stable expression plasmids in Cupriavidus necator H16 Publikation in PURE anzeigen
Dominic Goj, Stella Ebner, Melissa Horvat, Simon Arhar, Ludmila Martínková and Margit Winkler Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase Publikation in PURE anzeigen
Margit Winkler, Hannah Breuer and Lukas Schober Aldehyde Reductase activity of Carboxylic Acid Reductases Publikation in PURE anzeigen
Barbora Křístková, Ludmila Martínková, Lenka Rucká, Michael Kotik, Natalia Kulik, Robert Rädisch, Margit Winkler and Miroslav Pátek Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry Publikation in PURE anzeigen
Astrid Schiefer, Lukas Schober, Margit Winkler, Jacek Plewka and Florian Rudroff Oxidative cleavage of C=C double bonds using novel metal-dependent aromatic dioxygenases Publikation in PURE anzeigen
Jorge González-Rodriguez, Margit Winkler and Florian Rudroff Concurrent Cascade Synthesis of Pyrazines Relevant to Food Industry under Biocompatible Conditions Publikation in PURE anzeigen
Valentina Jurkas, Hana Dobiašová, Eva Puchľová, Marta Parego, Kvetoslava Vranková, Peter Both, Florian Rudroff, Fabio Parmeggiani and Margit Winkler Enzymatic cascade to natural ligustrazine Publikation in PURE anzeigen
Margit Winkler, Dominic Goj, Hannah Breuer, Melissa Horvat and L. Martinková Recent Advances on CARs Publikation in PURE anzeigen
Robert Kourist, Marianna Karava and Margit Winkler Hydrogen-driven biocatalytic reduction of carboxylic acids Publikation in PURE anzeigen
Hana Dobiašová, Valentina Jurkaš, Frederika Kabátová, Melissa Horvat, Florian Rudroff, Kvetoslava Vranková, Peter Both and Margit Winkler Carboligation towards production of hydroxypentanones Publikation in PURE anzeigen
Hana Dobiašová, Valentina Jurkas, Eva Puchľová, Kvetoslava Vranková, Peter Both, Florian Rudroff and Margit Winkler Unveiling novel carboligase homologues for C1-C3 Aldehyde conversion to bioactive hydroxy ketones Publikation in PURE anzeigen
Valentina Jurkas, Hana Dobiašová, Eva Puchľová, Marta Parego, Kvetoslava Vranková, Peter Both, Florian Rudroff, Fabio Parmeggiani and Margit Winkler Enzymatic cascade to natural ligustrazine Publikation in PURE anzeigen
Jorge González-Rodriguez, Eva Puchľová, Maren Podewitz, Fabio Parmeggiani, Margit Winkler, Peter Both, Florian Rudroff and Peter Šiška Biocatalytic Cascade for the Selective Synthesis of Asymmetric Pyrazines Publikation in PURE anzeigen
Jorge González-Rodriguez, Eva Puchľová, Maren Podewitz, Fabio Parmeggiani, Margit Winkler, Peter Both, Florian Rudroff and Peter Šiška Biocatalytic Cascade for the Selective Synthesis of Asymmetric Pyrazines Publikation in PURE anzeigen
Margit Winkler, Holly Stolterfoht, Zvjezdana Findrik Blažević and Robert Kourist Carboxylic acid Reductase: towards understanding of bottlenecks Publikation in PURE anzeigen
Margit Winkler CirculH2-Sustainable Production of Chemicals Publikation in PURE anzeigen
Margit Winkler, Lukas Schober, Astrid Schiefer, Sven Heimhilcher, Jacek Plewka and Florian Rudroff Enzymatic C=C bond cleavage: characterization and engineering of a new dioxygenase Publikation in PURE anzeigen
Dominic Goj, Karin Reicher, Claudia Rinnofner and Margit Winkler Ethanol driven protein expression in K. phaffii Publikation in PURE anzeigen
Holly Stolterfoht, Margit Winkler, Zvjezdana Findrik Blažević and Robert Kourist Carboxylic acid Reductase: towards understanding of bottlenecks Publikation in PURE anzeigen
Valentina Jurkas, Hana Dobiašová, Eva Puchľová, Marta Parego, Kvetoslava Vranková, Peter Both, Florian Rudroff, Fabio Parmeggiani and Margit Winkler Enzymatic cascade to natural ligustrazine Publikation in PURE anzeigen
Jorge González-Rodriguez, Eva Puchľová, Maren Podewitz, Fabio Parmeggiani, Margit Winkler, Peter Both, Florian Rudroff and Peter Šiška Biocatalytic Cascade for the Selective Synthesis of Asymmetric Pyrazines Publikation in PURE anzeigen
Margit Winkler and Ludmila Martínková Entwicklung einer zyanidfreien Kaskadenreaktion Publikation in PURE anzeigen
2023
Margit Winkler, Melissa Horvat, Astrid Schiefer, Victoria Weilch, Florian Rudroff, Miroslav Patek and Ludmila Martínková Organic Acid to Nitrile: a chemoenzymatic three-step route Publikation in PURE anzeigen
Barbora Kristkova, Robert Rädisch, Natalia Kulik, Melissa Horvat, Lenka Rucká, Michal Grulich, Florian Rudroff, Alan Kadek, Miroslav Patek, Margit Winkler and Ludmila Martínková Scanning aldoxime dehydratase sequence space and characterization of a new aldoxime dehydratase OxdFv from Fusarium vanettenii Publikation in PURE anzeigen
Katharina Ebner, Lukas Pfeifenberger, Veronika Schusterbauer, Claudia Rinnofner, Anton Glieder and Margit Winkler Discovery and heterologous expression of unspecific Peroxygenases Publikation in PURE anzeigen
Douglas Weber, Lucas de Souza Bastos, Margit Winkler, Yeke Ni, Abil E. Aliev, Helen C. Hailes and Dörte Rother Multi-enzyme catalysed processes using purified and whole-cell biocatalysts towards a 1,3,4-substituted tetrahydroisoquinoline Publikation in PURE anzeigen
H Dobiašová, Valentina Jurkaš, Eva Puchľová, Florian Rudroff, Kvetoslava Vranková and Margit Winkler Carboligases catalysing the production of odorous α-hydroxy pentanones: Flavour of cheese, wine and durian Publikation in PURE anzeigen
Margit Winkler, Bastian Daniel, Chiam Hashem, Ludmila Martínková, Tea Pavkov-Keller and Hannah Breuer Carbonyl Reduction by Carboxylic Acid Reductases: An unsurprising surprise? Publikation in PURE anzeigen
Valentina Jurkaš, H Dobiašová, Kvetoslava Vranková, Dörte Rother, Florian Rudroff and Margit Winkler Cheese, wine and durian flavour components produced by carboligases Publikation in PURE anzeigen
Lukas Schober, Astrid Schiefer, Florian Rudroff and Margit Winkler Diversification of carotenoid cleaving enzymes serving as 'ozonylases' for generation of aldehydes from a lignin-based feedstock Publikation in PURE anzeigen
Astrid Schiefer, Lukas Schober, Margit Winkler and Florian Rudroff Biocatalytic oxidative cleavage of alkenes using novel metal-dependent aromatic dioxygenases Publikation in PURE anzeigen
Stefan Giparakis, Margit Winkler and Florian Rudroff Nature goes natural: A novel chemo-enzymatic one-pot cascade for the synthesis of fragrance & flavor aldehydes Publikation in PURE anzeigen
Barbora Kristkova, Ludmila Martínková, Robert Rädisch, Lenka Rucká, Natalia Kulik, Margit Winkler and Miroslav Pátek Activity, stability and applications of aldoxime dehydratase biocatalysts immobilized on metal affinity resins Publikation in PURE anzeigen
Marianna Karava, Sara Xhemalaj, Vera Lambauer, Regina Kratzer, Margit Winkler and Robert Kourist H2 and CO2 fueled reduction of carboxylic acids in Cupriavidus necator Publikation in PURE anzeigen
Carsten Pichler, Kay Domenico Novak, Astrid Weninger, Christoph Reisinger, Claudia Rinnofner, Lukas Pfeifenberger, Katharina Ebner, Margit Winkler and Anton Glieder More enzymes more opportunities for oxyfunctionalization: Sixty novel unspecific peroxygenases for biocatalysis Publikation in PURE anzeigen
Alexandra Deibuk, Sandra Flecker, Karin Reicher, Claudia Rinnofner and Margit Winkler Methanol-free enzyme production using Pichia pastoris Publikation in PURE anzeigen
Andrea Hönikl, Maja Mollat, Margit Winkler, Morten Sørlie, Anton Glieder and Lukas Rieder Getting active: LPMOs made by Komagataella phaffii Publikation in PURE anzeigen
Lukas Rieder, Andrea Hönikl, Vanessa Holzschuster, Aline Rindler, Dorina Sebe, Margit Winkler, Tomasz Borowski, Artur Góra, Morten Sørlie and Anton Glieder Copper enzymes for sustainable, modern chemistry Publikation in PURE anzeigen
Andrea Hönikl, Margit Winkler, Anton Glieder and Lukas Rieder The more the better: Unveiling the secrets of LPMO production in Komagataella phaffii Publikation in PURE anzeigen
Stefan Giparakis, Margit Winkler and Florian Rudroff Nature stays natural: Two novel chemo-enzymatic one-pot cascades for the synthesis of fragrance & flavor aldehydes Publikation in PURE anzeigen
2022
Melissa Horvat, Victoria Weilch, Robert Rädisch, Sebastian Hecko, Astrid Schiefer, Florian Rudroff, Birgit Wilding, Norbert Klempier, Miroslav Patek, Ludmila Martínková and Margit Winkler Chemoenzymatic one-pot reaction from carboxylic acid to nitrile via oxime Publikation in PURE anzeigen
Masethabela Maphatsoe, Chiam Hashem, Jonathan Guyang Ling, Melissa Horvat, Karl Rumbold, Farah Diba Abu Bakar and Margit Winkler Characterization and immobilization of Pycnoporus cinnabarinus carboxylic acid reductase, PcCAR2 Publikation in PURE anzeigen
Manu Manjunath Kanti, Isabelle Striessnig-Bina, Beatrix Irene Wieser, Silvia Schauer, Gerd Leitinger, Thomas O. Eichmann, Martina Schweiger, Margit Winkler, Elke Winter, Andrea Lana, Iris Kufferath, Leigh Matthew Marsh, Grazyna Kwapiszewska, Rudolf Zechner, Gerald Hoefler and Paul Willibald Vesely Adipose triglyceride lipase mediated lipid catabolism is essential for bronchiolar regeneration Publikation in PURE anzeigen
Melissa Horvat, Astrid Schiefer, Florian Rudroff, Miroslav Patek, Ludmila Martínková and Margit Winkler One pot chemoenzymatic route from acid to nitrile Publikation in PURE anzeigen
Lukas Schober, Astrid Schiefer, Florian Rudroff and Margit Winkler Oxidative Alkene cleavage with the metal-dependent enzyme MapADO Publikation in PURE anzeigen
Stefan Giparakis, Margit Winkler and Florian Rudroff Nature goes natural: A novel chemo-enzymatic one-pot cascade for the synthesis of fragrance & flavor aldehydes Publikation in PURE anzeigen
Carsten Pichler, Kay Domenico Novak, Astrid Weninger, Christoph Reisinger, Claudia Rinnofner, Lukas Pfeifenberger, Katharina Ebner, Margit Winkler and Anton Glieder More enzymes more opportunities for oxyfunctionalization: Sixty novel unspecific peroxygenases for biocatalysis Publikation in PURE anzeigen
Barbora Kristkova, Robert Rädisch, Natalia Kulik, Melissa Horvat, Lenka Rucká, Miroslav Patek, Margit Winkler and Ludmila Martínková Occurrence and variability of aldoxime dehydratase with focus on enzyme OxdFv from Fusarium vanettenii Publikation in PURE anzeigen
Margit Winkler, Lukas Schober, Dominic Goj, Chiam Hashem and Florian Rudroff Expanding the toolbox for biocatalytic aldehyde synthesis Publikation in PURE anzeigen
Carsten Pichler, Kay Domenico Novak, Astrid Weninger, Christoph Reisinger, Claudia Rinnofner, Lukas Pfeifenberger, Katharina Ebner, Margit Winkler and Anton Glieder More enzymes more opportunities for oxyfunctionalization: Sixty novel unspecific peroxygenases for biocatalysis Publikation in PURE anzeigen
Lukas Schober, Astrid Schiefer, Florian Rudroff and Margit Winkler MapADO: Expression and Characterisation of a novel metal-dependent alkene cleaving enzyme Publikation in PURE anzeigen
Chiam Hashem, Julius Hochrinner, Moritz Benjamin Bürgler, Claudia Rinnofner, Harald Pichler and Margit Winkler From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii Publikation in PURE anzeigen
Margit Winkler, Lukas Schober, Dominic Goj, Chiam Hashem and Florian Rudroff Expanding the toolbox for biocatalytic aldehyde synthesis Publikation in PURE anzeigen
Margit Winkler, Birgit Grill, Karin Reicher, Helmut Schwab, Sebastian Arndt, Alexander Nauth, Georg Steinkellner, Kai Donsbach, Siegfried R. Waldvogel and Till Opatz Enzymatic dynamic kinetic resolution and ex-cell anodic oxidation for Levetiracetam synthesis Publikation in PURE anzeigen
Dominic Goj, Stella Ebner, Melissa Horvat and Margit Winkler Pichia pastoris as a producer of carboxylic acid reductases Publikation in PURE anzeigen
Valentina Jurkaš, H Dobiašová, Melissa Horvat, Kvetoslava Vranková, Florian Rudroff and Margit Winkler Flavour of cheese, wine and durian: synthesis of odorous α-hydroxy pentanones with carboligases Publikation in PURE anzeigen
Lukas Schober, Astrid Schiefer, Florian Rudroff and Margit Winkler Oxidative Alkene cleavage with novel metal-dependent carotenoid cleaving oxygenases as an alternative to ozonolysis Publikation in PURE anzeigen
Simon Arhar, Holly Stolterfoht-Stock, Thomas Rauter, Margit Winkler, Robert Kourist and Anita Emmerstorfer-Augustin On the way: Establishing CRISPR/Cas as genome editing tool in Cupriavidus necator H16 Publikation in PURE anzeigen
Carsten Pichler, Kay Domenico Novak, Astrid Weninger, Christoph Reisinger, Claudia Rinnofner, Lukas Pfeifenberger, Katharina Ebner, Margit Winkler and Anton Glieder More enzymes more opportunities for oxyfunctionalization: Sixty novel unspecific peroxygenases for biocatalysis Publikation in PURE anzeigen
Barbora Kristkova, Natalia Kulik, Robert Rädisch, Lenka Rucká, Florian Rudroff, Miroslav Patek, Margit Winkler and Ludmila Martínková Search for aldoxime dehydratases for a new chemoenzymatic method of nitrile synthesis Publikation in PURE anzeigen
Bastian Daniel, Chiam Hashem, Marlene Leithold, Theo Sagmeister, Adrian Tripp, Holly Stolterfoht-Stock, Julia Messenlehner, Ronan Keegan, Christoph K. Winkler, Jonathan Guyang Ling, Sabry H.H. Younes, Gustav Oberdorfer, Farah Diba Abu Bakar, Karl Gruber, Tea Pavkov-Keller and Margit Winkler Structure of the Reductase Domain of a fungal Carboxylic Acid Reductase and its Substrate Scope in Thioester and Aldehyde Reduction Publikation in PURE anzeigen
Bastian Daniel, Chiam Hashem, Marlene Leithold, Theo Sagmeister, Adrian Tripp, Holly Stolterfoht-Stock, Julia Messenlehner, Ronan Keegan, Christoph K. Winkler, Jonathan Guyang Ling, Sabry H.H. Younes, Gustav Oberdorfer, Farah Diba Abu Bakar, Karl Gruber, Tea Pavkov-Keller and Margit Winkler Structure and function of the reductase domain of the Neurospora crassa carboxylic acid reductase Publikation in PURE anzeigen
2021
Anna Schwarz, Sebastian Hecko, Florian Rudroff, Jeffrey Kohrt, Roger Howard and Margit Winkler Cell-free in vitro reduction of carboxylates to aldehydes: with crude enzyme preparations to a key pharmaceutical building block Publikation in PURE anzeigen
Douglas Weber, D. Patsch, Annikka Neumann, Margit Winkler and Dörte Rother Production of the Carboxylate Reductase from Nocardia otitidiscaviarum in a Soluble, Active Form for in vitro Applications Publikation in PURE anzeigen
Melissa Horvat and Margit Winkler Whole cell Production of fatty aldehydes by Mycobacterium marinum Carboxylate reductase Publikation in PURE anzeigen
Chiam Hashem, Stefan Steinberger, Holly Stolterfoht, Claudia Rinnofner, Margit Winkler and Harald Pichler Approaching an Efficient System for Green Leaf Volatile Synthesis Publikation in PURE anzeigen
Sebastian Arndt, Birgit Grill, Helmut Schwab, Georg Steinkellner, Dominik Weis, Urska Pogorevcnik, Alexander Nauth, Karl Gruber, Till Opatz, Kai Donsbach, Siegfried R. Waldvogel and Margit Winkler The sustainable synthesis of levetiracetam by an enzymatic dynamic kinetic resolution and an ex-cell anodic oxidation Publikation in PURE anzeigen
Masethabela Maphatsoe, Jonathan Guyang Ling, Chiam Hashem, Melissa Horvat, Farah Diba Abu Bakar, Margit Winkler and Karl Rumbold Elucidating the role of a Pycnoporous cinnabarinus carboxylic acid reductase, PcCAR2, as a potential biocatalyst Publikation in PURE anzeigen
Lukas Pfeifenberger, Alexander Svoboda, Katharina Ebner, Claudia Rinnofner, Anton Glieder, Wolfgang Kroutil and Margit Winkler Secretory Production of novel Peroxygenases in Pichia pastoris Publikation in PURE anzeigen
Margit Winkler, Melissa Horvat and Chiam Hashem With enzymes and microbes to flavor and fragrance molecules Publikation in PURE anzeigen
Daniel Schwendenwein, Anna K. Ressmann, Marcello Entner, Viktor Savic, Margit Winkler and Florian Rudroff Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes Publikation in PURE anzeigen
Birgit Grill, Melissa Horvat, Helmut Schwab, Ralf Gross, Kai Donsbach and Margit Winkler Gordonia hydrophobica Nitrile Hydratase for Amide preparation from nitriles Publikation in PURE anzeigen
Daniela Obitková, Melissa Horvat, Victoria Weilch, Robert Rädisch, Sebastian Hecko, Astrid Schiefer, Florian Rudroff, Miroslav Patek, Ludmila Martínková and Margit Winkler Chemoenzymatic synthesis of nitriles with carboxylic acid reductase and aldoxime dehydratase Publikation in PURE anzeigen
Birgit Grill, Melissa Horvat, Helmut Schwab, Ralf Gross, Kai Donsbach and Margit Winkler Gordonia hydrophobica Nitrile Hydratase for Amide Preparation from NitrilesEnzymes and Biocatalysis95-104 Publikation in PURE anzeigen
2020
Gernot Strohmeier, Anna Schwarz, Jennifer N. Andexer and Margit Winkler Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems Publikation in PURE anzeigen
Mihai-Silviu Tomescu, Deidre Davids, Matthew DuPlessis, Barbara Darnhofer, Ruth Birner-Gruenberger, Robert Archer, Daniel Schwendenwein, Gerhard Thallinger, Margit Winkler and Karl Rumbold High-throughput in-field bioprospecting for cyanogenic plants and hydroxynitrile lyases Publikation in PURE anzeigen
Birgit Grill, Maximilian Glänzer, Helmut Schwab, Steiner Kerstin, Daniel Pienaar, Dean Brady, Kai Donsbach and Margit Winkler Functional Expression and Characterization of a Panel of Cobalt and Iron-Dependent Nitrile Hydratases Publikation in PURE anzeigen
Melissa Horvat and Margit Winkler In vivo reduction of medium- to long-chain fatty acids by carboxylic acid reductase (CAR) enzymes: limitations and solutions Publikation in PURE anzeigen
Chiam Hashem, Holly Stolterfoht, Claudia Rinnofner, Stefan Steinberger, Margit Winkler and Harald Pichler Secretion of Pseudomonas Aeruginosa Lipoxygenase by Pichia Pastoris upon Glycerol Feed Publikation in PURE anzeigen
Melissa Horvat, Tanja Saskia Larch, Florian Rudroff and Margit Winkler Amino benzamidoxime (ABAO) based assay to identify efficient aldehyde-producing Pichia pastoris clones Publikation in PURE anzeigen
Yating Hu, Zhiwei Zhu, David Gradischnig, Margit Winkler, Jens Nielsen and Verena Siewers Engineering carboxylic acid reductase for selective synthesis of medium-chain fatty alcohols in yeast Publikation in PURE anzeigen
Team Leader
image/svg+xml
Margit Winkler
Dipl.-Ing. Dr.techn.