The Glieder team innovates protein expression for enzyme and pathway expression and engineering. Building on a strong platform of microbial expression systems with freedom to operate (FTO), advanced tools and methods for gene expression are developed. Synthetic biology approaches are applied to tune and balance (co-) expression of several proteins, for example to maximize the catalytic activity of cytochrome P450 whole cell catalysts, folding and secretion of enzymes, which are difficult to express. Engineering of expression systems includes the design and construction of synthetic promoters, terminators and expression vectors as well as the reengineering of microbial chassis strains. Applicable applied research is a top priority and therefore the construction of genetically stable expression strains is a core interest and activity. Discovery and Engineering of robust enzymes is applied for industrial biocatalyst development and to adapt enzymes for synthetic pathways. Most of these studies are dedicated to genes from eukaryotes, which are difficult to express in E. coli.