Glieder Team

Glieder Group - click to enlarge
Glieder Team - click to enlarge

Research Goals

The Glieder team innovates protein expression for enzyme and pathway expression and engineering. Building on a strong platform of microbial expression systems with freedom to operate (FTO), advanced tools and methods for gene expression are developed. Synthetic biology approaches are applied to tune and balance (co-) expression of several proteins, for example to maximize the catalytic activity of cytochrome P450 whole cell catalysts, folding and secretion of enzymes, which are difficult to express. Engineering of expression systems includes the design and construction of synthetic promoters, terminators and expression vectors as well as the reengineering of microbial chassis strains. Applicable applied research is a top priority and therefore the construction of genetically stable expression strains is a core interest and activity. Discovery and Engineering of robust enzymes is applied for industrial biocatalyst development and to adapt enzymes for synthetic pathways. Most of these studies are dedicated to genes from eukaryotes, which are difficult to express in E. coli.

Current Projects

  • November 2012 - September 2016
    IMI CHEM21/WP4 - Synthetic Biology
    Project Website

    WP4 of Chemical Manufacturing Methods for the 21st Century Pharmaceutical Industry is focusing on the transformation of simple carbon sources into high value products by microorganisms. This will be achieved by the design of efficient biosynthetic pathways in industrially relevant platform microorganism that allow the sustainable and economic production of complex molecules in order to reduce the number or even eliminate the necessity of synthesis steps by organic chemistry in production processes of small molecule drugs.

     

  • Ongoing
    Pichia Pool - FTO Pichia pastoris expression strains and vectors

    In a collaboration between TU Graz, the research Centre Applied Biocatalysis (now acib GmbH) and VTU Technology a platform of Pichia pastoris (Komagataella phaffi) strains and vectors for recombinant protein expression was generated to facilitate industrial research collaborations. This platform of tools is continuously improved to facilitate cloning and genomic information about these strains.


  • December 2012 - November 2015
    FP7 Project KYROBIO - Grant agreement no: 289646

    The Glieder Group provides innovative expression and fermentation strategies for enzyme production for the partners of this project on “The discovery, development and demonstration of biocatalysts for use in the industrial synthesis of chiral chemicals”. The major focus is on methanol independent production of hydroxynitrile lyases in tight collaboration with VTU technology, Ingenza and acib GmbH.


  • April 2015 - March 2019
    Horizon2020 Project ROBOX - Grant agreement #635734
    Project Website

    The TU Graz team contributes to this Horizon2020 project (Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols) by providing new and robust hydroxylation catalysts and innovative co-expression technologies for biocatalysis. Close interaction with industrial partners and lab scale demonstrations in small scale bioreactor experiments under reliable and controlled conditions secure the transferability of the research findings to participating industries. Locally the TUG team closely collaborates with Prof. Rolf Breinbauer and Dr. Margit Winkler from acib to stabilize whole cell biocatalyst preparations for the transfer to other labs and 
to apply and characterize the new catalysts in robust biotransformations. Internationally the research activities are closely linked with the research of Prof. Mattevi (Uni Pavia), Prof. Schwaneberg (RWTH), DSM and the Autonomous University Barcelona.


  • Ongoing
    Synthetic biology for gene expression in yeasts

    This work includes the design of synthetic parts for tunable gene expression in yeasts (Saccharomyces cerevisiae, Pichia pastoris, Schizosaccharomyces pombe) and the application of these new tools to generate new yeast chassis strains. Scale up in bioreactors is performed in collaboration with Prof. Herwig and Doz. Spadiut from TU Vienna and Prof. Kovar (ZHAW, CH)


  • January 2015 - December 2017
    acib industrial project Area 2 - New enzymes for polymer modification
    Project Website

    The in silico screening of genomic databases and own next generation sequencing data is applied to identify novel enzymes acting on polymers and for polymer synthesis. The identified biocatalysts for polymer processing are then engineered for improved technological features.


  • 2015 - 2017
    acib industrial project Area 4
    Project Website

    Enzyme and reaction engineering for the biocatalytic production of carboxylic acid derivatives for API manufacturing in close collaboration with Prof. Wolfgang Kroutil and Prof. Kurt Faber (University Graz).


  • 2015-2016
    PRIZE - Prototypenfoerderung (AWS)
    Project Website

    Applying technologies from recent patent applications by TU Graz, the production of horseradish peroxidase isoenzymes for diagnostic, biocatalytic and waste water treatment applications will be improved and demonstrated.

Current Theses

  • MSc. Astrid Weninger: Engineering and production of robust oxygenases
  • MSc. Lukas Sturmberger: Discovery, Engineering and Expression of novel polymer modifying Enzymes
  • MSc. Fitri Nurfitriani: OEAD fellow: Tunable synthetic Pichia pastoris promoters
  • MSc. Julia Pitzer: industrial project
  • MSc. Thomas Kickenweiz: ARAP fellowship, collaboration with ICES (Dr. WU), A*Star, Singapore
  • Rui Portella: Synthetic core promoter design jointly supervised with Prof. Rui Oliveira from FCT Portugal

Completed Theses

  • Mag. Roland Weis: High throughput methods for protein engineering with the host system Pichia pastoris
  • Mag. Alexander Kern: Strategic transformation of key genes from an Aspergillus niger citric acid production strain into Pichia pastoris
  • MSc Liu Zhibin: Directed Evolution of Industrial enzymes employing Pichia pastoris
  • DI Franz Hartner: Engineering Pichia pastoris for whole cell biotransformation
  • DI Mathias schittmayer: New Oxidoreductases for metabolite synthesis
  • Mag. Ulrike Schreiner: Molecular engineering of nitrilases
  • MSc Aleksandra Andyushkova (Fuchs): Establishing fission yeast as a host for eukaryotic genomic libraries
  • DI Maria Freigassner: Alternative Oxidases: Elucidation of structural differences between plants and fungi
  • Mag. Brigitte Höller: New Oxidoreductases for Biocatalysis
  • DI Sandra Abad: New strategies and tools for protein expression and engineering using Pichia pastoris
  • DI Claudia Ruth: Expression of recombinant proteins in Pichia pastoris employing synthetic promoters
  • DI Beate Pscheidt: Directed Evolution of PaHNL5
  • DI Martina Geier: Redesign of Oxygenases for Biocatalysis
  • DI Andreas Braun: Expression of human liver cytochrome P450 enzymes in the non conventional yeast Yarrowia lipolytica
  • DI Andrea Mellitzer: Expression and Engineering of eukaryotic enzymes for polymer modification using Pichia pastoris
  • MSc Laura Hannele Naeaetsaari: Horseradish peroxidase discovery and production in a new improved Pichia pastoris expression system
  • MSc Aleksandra Mitrovic: Discovery and optimization of fungal lignocellulolytic enzymes
  • DI Thomas Vogl: Synthetic biology to improve protein expression in Pichia pastoris
  • Florian Krainer: Production and characterization of recombinant plant peroxidases

Selected Publications

2024
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen and Anton Glieder Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii Publikation in PURE anzeigen
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder and Xavier Garcia-Ortega Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection Publikation in PURE anzeigen
Julian C. Krappinger, Carla M. Aguilar Gomez, Andrea Hoenikl, Veronika Schusterbauer, Anna Maria Hatzl, Julia Feichtinger and Anton Glieder dMAD7 is a promising tool for targeted gene regulation in the methylotrophic yeast Komagataella phaffii Publikation in PURE anzeigen
2023
Julia Jodlbauer, Lukas Rieder, Anton Glieder and Birgit Wiltschi Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coliMethods in molecular biology75-86 Publikation in PURE anzeigen
2022
Matic Srdič, Nico D Fessner, Deniz Yildiz, Anton Glieder, Markus Spiertz and Ulrich Schwaneberg Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor Publikation in PURE anzeigen
Nico D Fessner, Hansjörg Weber and Anton Glieder Regiospecific 7-hydroxylation of ten-carbon monoterpenes by detoxifying CYP5035S7 monooxygenase of the white-rot fungus Polyporus arcularius Publikation in PURE anzeigen
Veronika Schusterbauer, Jasmin E. Fischer, Sarah Gangl, Lisa Schenzle, Claudia Rinnofner, Martina Geier, Christian Sailer, Anton Glieder and Gerhard G. Thallinger Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in Komagataella phaffii: A Budding Yeast in Distress Publikation in PURE anzeigen
Nico D. Fessner, Hansjoerg Weber and Anton Glieder Regioselective Hydroxylation of Stilbenes by White-Rot Fungal P450s Enables Preparative-Scale Synthesis of Stilbenoids Publikation in PURE anzeigen
Vitalijs Zubkovs, Hanxuan Wang, Nils Schuergers, Astrid Weninger, Anton Glieder, Stefano Cattaneo and Ardemis A. Boghossian Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring Publikation in PURE anzeigen
Julia Pitzer, Kerstin Steiner, Christian Schmid, Viktor K. Schein, Christoph Prause, Claudia Kniely, Michaela Reif, Martina Geier, Elena Pietrich, Tamara Reiter, Philipp Selig, Clemens Stückler, Peter Pöchlauer, Georg Steinkellner, Karl Gruber, Helmut Schwab, Anton Glieder and Wolfgang Kroutil Racemization-free and scalable amidation of l-proline in organic media using ammonia and a biocatalyst only Publikation in PURE anzeigen
Nico D. Fessner, Christopher Grimm, Matic Srdič, Hansjörg Weber, Wolfgang Kroutil, Ulrich Schwaneberg and Anton Glieder Natural Product Diversification by One-Step Biocatalysis using Human P450 3A4 Publikation in PURE anzeigen
Jasmin Elgin Fischer and Anton Glieder CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) GenomeYeast Metabolic Engineering121-133 Publikation in PURE anzeigen
2021
Nico D Fessner, Christopher Grimm, Wolfgang Kroutil and Anton Glieder Late-Stage Functionalisation of Polycyclic (N-Hetero-) Aromatic Hydrocarbons by Detoxifying CYP5035S7 Monooxygenase of the White-Rot Fungus Polyporus arcularius Publikation in PURE anzeigen
Laura Rotilio, Alexander Swoboda, Katharina Ebner, Claudia Rinnofner, Anton Glieder, Wolfgang Kroutil and Andrea Mattevi Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst Publikation in PURE anzeigen
Nico D Fessner, David R Nelson and Anton Glieder Evolution and enrichment of CYP5035 in Polyporales Publikation in PURE anzeigen
Lukas Rieder, Katharina Ebner, Anton Glieder and Morten Sørlie Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris Publikation in PURE anzeigen
Javier Garrigós-Martínez, Astrid Weninger, José Luis Montesinos-Seguí, Christian Schmid, Francisco Valero, Claudia Rinnofner, Anton Glieder and Xavier Garcia-Ortega Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9 Publikation in PURE anzeigen
Javier Garrigós-Martínez, Kiira Vuoristo, Miguel Angel Nieto-Taype, Juha Tähtiharju, Jaana Uusitalo, Pauliina Tukiainen, Christian Schmid, Ilya Tolstorukov, Knut Madden, Merja Penttilä, José Luis Montesinos-Seguí, Francisco Valero, Anton Glieder and Xavier Garcia-Ortega Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris Publikation in PURE anzeigen
2020
Patricia Gomez De Santos, Sofia Lazaro, Javier Vinã-Gonzalez, Manh Dat Hoang, Israel Sánchez-Moreno, Anton Glieder, Frank Hollmann and Miguel Alcalde Evolved Peroxygenase-Aryl Alcohol Oxidase Fusions for Self-Sufficient Oxyfunctionalization Reactions Publikation in PURE anzeigen
Nico D. Fessner, Matic Srdič, Hansjörg Weber, Christian Schmid, David Schönauer, Ulrich Schwaneberg and Anton Glieder Preparative-Scale Production of Testosterone Metabolites by Human Liver Cytochrome P450 Enzyme 3A4 Publikation in PURE anzeigen
Thomas Vogl, Jasmin Elgin Fischer, Patrick Hyden, Richard Wasmayer, Lukas Sturmberger and Anton Glieder Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris Publikation in PURE anzeigen
2019
Claudia Rinnofner, Bianca Kerschbaumer, Hansjörg Weber, Anton Glieder and Margit Winkler Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst Publikation in PURE anzeigen
Jasmin Elgin Fischer, Anna-Maria Hatzl, Astrid Weninger, Christian Schmid and Anton Glieder Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies Publikation in PURE anzeigen
Hana Raschmanová, Iwo Zamora, Martina Borčinová, Patrick Meier, Astrid Weninger, Dominik Mächler, Anton Glieder, Karel Melzoch, Zdeněk Knejzlík and Karin Kovar Single-cell approach to monitor the unfolded protein response during biotechnological processes with Pichia pastoris Publikation in PURE anzeigen
Lukas Rieder, Nico Teuschler, Katharina Ebner and Anton Glieder Eukaryotic Expression Systems for Industrial EnzymesIndustrial Enzyme Applications47-69 Publikation in PURE anzeigen
2018
Elisa Lanfranchi, Birgit Grill, Zainab Raghoebar, Sander Van Pelt, Roger A Sheldon, Kerstin Steiner, Anton Glieder and Margit Winkler Production of Hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and Its Immobilization as a CLEA to Generate a Robust Biocatalyst Publikation in PURE anzeigen
Thomas Kickenweiz, Anton Glieder and Jin Chuan Wu Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase Publikation in PURE anzeigen
Anna Katharina Migglautsch, Melissa Willim, Bettina Schweda, Anton Glieder, Rolf Breinbauer and Margit Winkler Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X Publikation in PURE anzeigen
Filippo Fiorentini, Anna-Maria Hatzl, S Schmidt, Simone Savino, Anton Glieder and Andrea Mattevi The Extreme Structural Plasticity in the CYP153 Subfamily of P450s Directs Development of Designer Hydroxylases Publikation in PURE anzeigen
Joseph Hosford, Morgane Valles, Florian W. Krainer, Anton Glieder and Lu Shin Wong Parallelized biocatalytic scanning probe lithography for the additive fabrication of conjugated polymer structures Publikation in PURE anzeigen
Thomas Vogl, Thomas Kickenweiz, Julia Pitzer, Lukas Sturmberger, Astrid Weninger, Bradley W Biggs, Eva-Maria Köhler, Armin Baumschlager, Jasmin Elgin Fischer, Patrick Hyden, Marlies Wagner, Martina Baumann, Nicole Borth, Martina Geier, Parayil Kumaran Ajikumar and Anton Glieder Engineered bidirectional promoters enable rapid multi-gene co-expression optimization Publikation in PURE anzeigen
2017
Elisa Lanfranchi, Tea Pavkov-Keller, Eva-Maria Koehler, Matthias Diepold, Kerstin Steiner, Barbara Darnhofer, Jürgen Hartler, Tom Van Den Bergh, Henk-Jan Joosten, Mandana Gruber-Khadjawi, Gerhard G Thallinger, Ruth Birner-Gruenberger, Karl Gruber, Margit Winkler and Anton Glieder Enzyme discovery beyond homology Publikation in PURE anzeigen
Rui M C Portela, Thomas Vogl, Katharina Ebner, Rui Oliveira and Anton Glieder Pichia pastoris Alcohol Oxidase 1 (AOX1) Core Promoter Engineering by High Resolution Systematic Mutagenesis Publikation in PURE anzeigen
Astrid Weninger, Jasmin Fischer, Hana Raschmanová, Claudia Kniely, Thomas Vogl and Anton Glieder Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers Publikation in PURE anzeigen
Kristína Markošová, Andrea Camattari, Michal Rosenberg, Anton Glieder, Nicholas J Turner and Martin Rebroš Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris Publikation in PURE anzeigen
Rui M C Portela, Thomas Vogl, Claudia Kniely, Jasmin E Fischer, Rui Oliveira and Anton Glieder Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species Publikation in PURE anzeigen
Elisa Lanfranchi, Tea Pavkov-Keller, Eva-Maria Koehler, Matthias Diepold, Kerstin Steiner, Barbara Darnhofer, Jürgen Hartler, Tom Van Den Bergh, Henk-Jan Joosten, Mandana Gruber-Khadjawi, Gerhard G Thallinger, Ruth Birner-Gruenberger, Karl Gruber, Margit Winkler and Anton Glieder Erratum Publikation in PURE anzeigen
2016
Katrin Weinhandl, Melanie Ballach, Margit Winkler, Ahmad Mudassar, Anton Glieder, Ruth Birner-Gruenberger, Ian Fotheringham and Franck Escalettes Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT) Publikation in PURE anzeigen
Thomas Vogl, Lukas Sturmberger, Thomas Kickenweiz, Richard Wasmayer, Christian Schmid, Anna-Maria Hatzl, Michaela Agnes Gerstmann, Julia Pitzer, Marlies Wagner, Gerhard G Thallinger, Martina Geier and Anton Glieder A Toolbox of Diverse Promoters Related to Methanol Utilization Publikation in PURE anzeigen
Florian W. Krainer, Michaela A. Gerstmann, Barbara Darnhofer, Ruth Birner-Gruenberger and Anton Glieder Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris Publikation in PURE anzeigen
Astrid Weninger, Anna-Maria Hatzl, Christian Schmid, Thomas Vogl and Anton Glieder Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris Publikation in PURE anzeigen
Lukas Sturmberger, Paal W Wallace, Anton Glieder and Ruth Birner-Gruenberger Synergism of proteomics and mRNA sequencing for enzyme discovery Publikation in PURE anzeigen
Florian Krainer, Barbara Darnhofer, Ruth Birner-Gruenberger and Anton Glieder Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris Publikation in PURE anzeigen
2015
Martina Geier, Pia Carina Fauland, Thomas Vogl and Anton Glieder Compact multi-enzyme pathways in P. pastoris Publikation in PURE anzeigen
Florian Krainer and Anton Glieder An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Publikation in PURE anzeigen
Thomas Vogl, Mudassar Ahmad, Florian Krainer, Helmut Schwab and Anton Glieder Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris Publikation in PURE anzeigen
Elisa Lanfranchi, Eva-Maria Köhler, Barbara Darnhofer, Kerstin Steiner, Ruth Birner-Grünberger, Anton Glieder and Margit Winkler Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection Publikation in PURE anzeigen
Florian Krainer, Simona Capone, Martin Jäger, Thomas Vogl, Michaela Agnes Gerstmann, Anton Glieder, Christoph Herwig and Oliver Spadiut Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris. Publikation in PURE anzeigen
Martina Geier, Christoph Brandner, Gernot A Strohmeier, Mélanie Hall, Franz Stefan Hartner and Anton Glieder Engineering Pichia pastoris for improved NADH regeneration Publikation in PURE anzeigen
Astrid Weninger, Anton Glieder and Thomas Vogl A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris Publikation in PURE anzeigen
Magali Tanghe, Barbara Danneels, Andrea Camattari, Anton Glieder, Isabel Vandenberghe, Bart Devreese, Ingeborg Stals and Tom Desmet Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris Publikation in PURE anzeigen
Kerstin Steiner, Anton Glieder and Mandana Gruber Cyanohydrin Formation / Henry ReactionSience of Synthesis1-5 Publikation in PURE anzeigen
Julia Pitzer, Bob Van Hove, Aaron M. Love, Parayil Kumaran Ajikumar, Marjan De Mey and Anton Glieder Novel DNA and RNA elementsSynthetic Biology65-99 Publikation in PURE anzeigen
2014
Oliver Spadiut, Simona Capone, Florian Krainer, Anton Glieder and Christoph Herwig Microbials for the production of monoclonal antibodies and antibody fragments Publikation in PURE anzeigen
Laura Näätsaari, Florian Krainer, Michael Schubert, Anton Glieder and Gerhard G. Thallinger Peroxidase gene discovery from the horseradish transcriptome Publikation in PURE anzeigen
Aleksandra Mitrovic, Karlheinz Flicker, Georg Steinkellner, Karl Gruber, Christoph Reisinger, Christoph Schirrmacher, Andrea Camattari and Anton Glieder Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii Publikation in PURE anzeigen
Thomas Vogl, Gerhard Thallinger, Günther Zellnig, David Drew, James Cregg, Anton Glieder and Maria Elisabeth Freigaßner Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression Publikation in PURE anzeigen
Shuke Wu, Yongzheng Chen, Yi Xu, Aitao Li, Qisong Xu, Anton Glieder and Zhi Li Enantioselective trans-dihydroxylation of aryl olefins by cascade biocatalysis with recombinant escherichia coli coexpressing monooxygenase and epoxide hydrolase Publikation in PURE anzeigen
Andrea Mellitzer, Claudia Ruth, Claes Gustafson, Mark Welch, Ruth Birner-Gruenberger, Roland Weis, Thomas Purkarthoer and Anton Glieder Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks Publikation in PURE anzeigen
Barbara Petschacher, Nicole Staunig, Monika Mueller, Martin Schuermann, Daniel Mink, Stefaan Wildemann, Karl Gruber and Anton Glieder Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD (P)+ Regeneration in Biocatalytic Oxidations Publikation in PURE anzeigen
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Carbon source dependent promoters in yeasts Publikation in PURE anzeigen
Thomas Vogl, Claudia Ruth, Julia Pitzer, Thomas Kickenweiz and Anton Glieder Synthetic Core Promoters for Pichia pastoris Publikation in PURE anzeigen
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Extracellular transaminases for biocatalysisNew BiotechnologyS198-S198 Publikation in PURE anzeigen
Margit Winkler, Martina Geier, Thorsten Bachler, Steven P Hanlon, Matthias Kittelmann, Anton Glieder, Stephan Lütz and Beat Wirz Human flavin monooxygenase 2: Heterologous expression in E. coli and API modificationNew BiotechnologyS82-S82 Publikation in PURE anzeigen
Florian W. Krainer, Robert Pletzenauer, Laura Rossetti, Christoph Herwig, Anton Glieder and Oliver Spadiut Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris Publikation in PURE anzeigen
Aleksandra Mitrovic and Anton Glieder Discovery of fungal enzymes and pathwaysFungal Bio-Molecules: Sources, Applications and Recent Developments51-61 Publikation in PURE anzeigen
2013
Elisa Lanfranchi, Kerstin Steiner, Anton Glieder, Ivan Hajnal, Roger A. Sheldon, Sander van Pelt and Margit Winkler Mini-Review: Recent Developments in Hydroxynitrile Lyases for Industrial Biotechnology Publikation in PURE anzeigen
Thomas Vogl, Franz Stefan Hartner and Anton Glieder New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris Publikation in PURE anzeigen
Thomas Vogl and Anton Glieder Regulation of Pichia pastoris promoters and its consequences for protein production Publikation in PURE anzeigen
Florian W. Krainer, Christoph Gmeiner, Lukas Neutsch, Markus Windwarder, Robert Pletzenauer, Christoph Herwig, Friedrich Altmann, Anton Glieder and Oliver Spadiut Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris Publikation in PURE anzeigen
Martina Geier, Christian Schmid and Anton Glieder First functional expression of cytochrome P450 3A4 in Pichia pastoris Publikation in PURE anzeigen
Martina Geier, Andreas Braun, Patrick Fladischer, Piotr Stepniak, Florian Rudroff, Christoph Hametner, Marko Mihovilovic and Anton Glieder Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation Publikation in PURE anzeigen
2012
Birgit Wilding, Margit Winkler, Regina Kratzer, Barbara Petschacher, Anton Glieder and Norbert Klempier NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION Publikation in PURE anzeigen
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari A novel multi-enzymatic high throughput assay for transaminase activity Publikation in PURE anzeigen
Martina Geier, Andreas Braun, Anita Emmerstorfer, Harald Pichler and Anton Glieder Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study Publikation in PURE anzeigen
Christoph Sygmund, Alexander Gutmann, Iris Krondorfer, Magdalena Kujawa, Anton Glieder, Beate Pscheidt, Dietmar Haltrich, Clemens Peterbauer and Roman Kittl Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris Publikation in PURE anzeigen
Andreas Braun, Bettina Halwachs, Martina Geier, Katrin Weinhandl, Michael Guggemos, Jan Marienhagen, Anna Joel Ruff, Ulrich Schwaneberg, Daniel E. Torres Pazmiño, Vincent Rabin, Gerhard Thallinger and Anton Glieder MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes Publikation in PURE anzeigen
Andreas Braun, Martina Geier, Bruno Buehler, Andreas Schmid, Stephan Mauersberger and Anton Glieder Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes Publikation in PURE anzeigen
Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz and Margit Winkler Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale Publikation in PURE anzeigen
Florian Krainer, Christian Dietzsch, Tanja Hajek, Christoph Herwig, Oliver Spadiut and Anton Glieder Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway Publikation in PURE anzeigen
Laura Näätsaari, Beate Mistlberger, Claudia Ruth, Tanja Hajek, Franz Stefan Hartner and Anton Glieder Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology Publikation in PURE anzeigen
Andrea Mellitzer, Roland Weis, Anton Glieder and Karlheinz Flicker Expression of lignocellulolytic enzymes in Pichia pastoris Publikation in PURE anzeigen
Andrea Mellitzer, Anton Glieder, Roland Weis, Christoph Reisinger and Karlheinz Flicker Sensitive high-throughput screening for the detection of reducing sugars Publikation in PURE anzeigen
Margit Winkler, Anton Glieder and Kerstin Steiner C–X Bond Formation: Hydroxynitrile lyases: From Nature to ApplicationComprehensive Chirality350-371 Publikation in PURE anzeigen
2011
Andrea Hasmann, Anita Eberl, Konstantin Schneider, K.-U. Schoening, F. Kaufmann, Anton Glieder, J. Kovac, Artur Cavaco-Paulo, Eva Wehrschütz-Sigl and Georg Gübitz Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3 Publikation in PURE anzeigen
Andreas Küberl, Jessica Schneider, Gerhard Thallinger, Ingund Anderl, Daniel Wibberg, Tanja Hajek, Sebastian Jaenicke, Karina Brinkrolf, Alexander Goesmann, Rafael Szczepanowski, Alfred Pühler, Helmut Schwab, Anton Glieder and Harald Pichler High-quality genome sequence of Pichia pastoris CBS7435 Publikation in PURE anzeigen
Matthias Schittmayer, Anton Glieder, Michael Karl Uhl, Andreas Winkler, Simone Zach, Jörg Schrittwieser, Wolfgang Kroutil, Peter Macheroux, Karl Gruber, Spiros Kambourakis, J. David Rozzell and Margit Winkler Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones Publikation in PURE anzeigen
Sandra Abad, Jozef Nahalka, Margit Winkler, Gabriele Bergler, Robert Speight, Anton Glieder and Bernd Nidetzky HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS Publikation in PURE anzeigen
2010
Kirsten Schroer, Klaus Peter Luef, Franz Stefan Hartner, Anton Glieder and Beate Pscheidt Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation Publikation in PURE anzeigen
Ulrike Schreiner, Bianca Hecher, Sascha Obrowsky, Kerstin Waich, Norbert Klempier, Georg Steinkellner, Karl Gruber, J. David Rozzell, Anton Glieder and Margit Winkler Directed evolution of Alcaligenes faecalis nitrilase Publikation in PURE anzeigen
Sandra Abad, Jozef Nahalka, Gabriele Bergler, S. Alison Arnold, Robert Speight, I. Fotheringhan, Bernd Nidetzky and Anton Glieder Stepwise engineering of a Pichia pastoris D-amino acid whole cell catalyst Publikation in PURE anzeigen
Claudia Ruth and Anton Glieder Perspectives on synthetic promoters for biocatalysis and biotransformation Publikation in PURE anzeigen
Daniel E Torres Pazmiño, Margit Winkler, Anton Glieder and Marco W Fraaije Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications Publikation in PURE anzeigen
Sandra Abad, Kerstin Kitz, Astrid Hörmann, Ulrike Schreiner, Franz Stefan Hartner and Anton Glieder Real time PCR based determination of gene copy numbers in Pichia pastoris Publikation in PURE anzeigen

Team Leader
image/svg+xml
Anton Glieder
Ao.Univ.-Prof. Mag.rer.nat. Dr.rer.nat.