Glieder Team

Glieder Group - click to enlarge
Glieder Team - click to enlarge

Research Goals

The Glieder team innovates protein expression for enzyme and pathway expression and engineering. Building on a strong platform of microbial expression systems with freedom to operate (FTO), advanced tools and methods for gene expression are developed.

Synthetic biology approaches are applied to tune and balance (co-) expression of several proteins, for example to maximize the catalytic activity of cytochrome P450 whole cell catalysts, folding and secretion of enzymes, which are difficult to express. Engineering of expression systems includes the design and construction of synthetic promoters, terminators and expression vectors as well as the reengineering of microbial chassis strains.

Applicable applied research is a top priority and therefore the construction of genetically stable expression strains is a core interest and activity. Discovery and Engineering of robust enzymes is applied for industrial biocatalyst development and to adapt enzymes for synthetic pathways. Most of these studies are dedicated to genes from eukaryotes, which are difficult to express in E. coli.


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Current Projects

  • November 2012 - September 2016
    IMI CHEM21/WP4 - Synthetic Biology
    Project Website

    WP4 of Chemical Manufacturing Methods for the 21st Century Pharmaceutical Industry is focusing on the transformation of simple carbon sources into high value products by microorganisms. This will be achieved by the design of efficient biosynthetic pathways in industrially relevant platform microorganism that allow the sustainable and economic production of complex molecules in order to reduce the number or even eliminate the necessity of synthesis steps by organic chemistry in production processes of small molecule drugs.

     

  • Ongoing
    Pichia Pool - FTO Pichia pastoris expression strains and vectors

    In a collaboration between TU Graz, the research Centre Applied Biocatalysis (now acib GmbH) and VTU Technology a platform of Pichia pastoris (Komagataella phaffi) strains and vectors for recombinant protein expression was generated to facilitate industrial research collaborations. This platform of tools is continuously improved to facilitate cloning and genomic information about these strains.


  • December 2012 - November 2015
    FP7 Project KYROBIO - Grant agreement no: 289646

    The Glieder Group provides innovative expression and fermentation strategies for enzyme production for the partners of this project on “The discovery, development and demonstration of biocatalysts for use in the industrial synthesis of chiral chemicals”. The major focus is on methanol independent production of hydroxynitrile lyases in tight collaboration with VTU technology, Ingenza and acib GmbH.


  • April 2015 - March 2019
    Horizon2020 Project ROBOX - Grant agreement #635734
    Project Website

    The TU Graz team contributes to this Horizon2020 project (Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols) by providing new and robust hydroxylation catalysts and innovative co-expression technologies for biocatalysis. Close interaction with industrial partners and lab scale demonstrations in small scale bioreactor experiments under reliable and controlled conditions secure the transferability of the research findings to participating industries. Locally the TUG team closely collaborates with Prof. Rolf Breinbauer and Dr. Margit Winkler from acib to stabilize whole cell biocatalyst preparations for the transfer to other labs and 
to apply and characterize the new catalysts in robust biotransformations. Internationally the research activities are closely linked with the research of Prof. Mattevi (Uni Pavia), Prof. Schwaneberg (RWTH), DSM and the Autonomous University Barcelona.


  • Ongoing
    Synthetic biology for gene expression in yeasts

    This work includes the design of synthetic parts for tunable gene expression in yeasts (Saccharomyces cerevisiae, Pichia pastoris, Schizosaccharomyces pombe) and the application of these new tools to generate new yeast chassis strains. Scale up in bioreactors is performed in collaboration with Prof. Herwig and Doz. Spadiut from TU Vienna and Prof. Kovar (ZHAW, CH)


  • January 2015 - December 2017
    acib industrial project Area 2 - New enzymes for polymer modification
    Project Website

    The in silico screening of genomic databases and own next generation sequencing data is applied to identify novel enzymes acting on polymers and for polymer synthesis. The identified biocatalysts for polymer processing are then engineered for improved technological features.


  • 2015 - 2017
    acib industrial project Area 4
    Project Website

    Enzyme and reaction engineering for the biocatalytic production of carboxylic acid derivatives for API manufacturing in close collaboration with Prof. Wolfgang Kroutil and Prof. Kurt Faber (University Graz).


  • 2015-2016
    PRIZE - Prototypenfoerderung (AWS)
    Project Website

    Applying technologies from recent patent applications by TU Graz, the production of horseradish peroxidase isoenzymes for diagnostic, biocatalytic and waste water treatment applications will be improved and demonstrated.


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Current Theses

  • MSc. Astrid Weninger: Engineering and production of robust oxygenases
  • MSc. Lukas Sturmberger: Discovery, Engineering and Expression of novel polymer modifying Enzymes
  • MSc. Fitri Nurfitriani: OEAD fellow: Tunable synthetic Pichia pastoris promoters
  • MSc. Julia Pitzer: industrial project
  • MSc. Thomas Kickenweiz: ARAP fellowship, collaboration with ICES (Dr. WU), A*Star, Singapore
  • Rui Portella: Synthetic core promoter design jointly supervised with Prof. Rui Oliveira from FCT Portugal

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Completed Theses

  • Mag. Roland Weis: High throughput methods for protein engineering with the host system Pichia pastoris
  • Mag. Alexander Kern: Strategic transformation of key genes from an Aspergillus niger citric acid production strain into Pichia pastoris
  • MSc Liu Zhibin: Directed Evolution of Industrial enzymes employing Pichia pastoris
  • DI Franz Hartner: Engineering Pichia pastoris for whole cell biotransformation
  • DI Mathias schittmayer: New Oxidoreductases for metabolite synthesis
  • Mag. Ulrike Schreiner: Molecular engineering of nitrilases
  • MSc Aleksandra Andyushkova (Fuchs): Establishing fission yeast as a host for eukaryotic genomic libraries
  • DI Maria Freigassner: Alternative Oxidases: Elucidation of structural differences between plants and fungi
  • Mag. Brigitte Höller: New Oxidoreductases for Biocatalysis
  • DI Sandra Abad: New strategies and tools for protein expression and engineering using Pichia pastoris
  • DI Claudia Ruth: Expression of recombinant proteins in Pichia pastoris employing synthetic promoters
  • DI Beate Pscheidt: Directed Evolution of PaHNL5
  • DI Martina Geier: Redesign of Oxygenases for Biocatalysis
  • DI Andreas Braun: Expression of human liver cytochrome P450 enzymes in the non conventional yeast Yarrowia lipolytica
  • DI Andrea Mellitzer: Expression and Engineering of eukaryotic enzymes for polymer modification using Pichia pastoris
  • MSc Laura Hannele Naeaetsaari: Horseradish peroxidase discovery and production in a new improved Pichia pastoris expression system
  • MSc Aleksandra Mitrovic: Discovery and optimization of fungal lignocellulolytic enzymes
  • DI Thomas Vogl: Synthetic biology to improve protein expression in Pichia pastoris
  • Florian Krainer: Production and characterization of recombinant plant peroxidases

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Selected Publications

2017
Elisa Lanfranchi, Tea Pavkov-Keller, Eva-Maria Koehler, Matthias Diepold, Kerstin Steiner, Barbara Darnhofer, Jürgen Hartler, Tom Van Den Bergh, Henk Jan Joosten, Mandana Gruber-Khadjawi, Gerhard Thallinger, Ruth Birner-Gruenberger, Karl Gruber, Margit Winkler and Anton Glieder ENZYME DISCOVERY BEYOND HOMOLOGY: A UNIQUE HYDROXYNITRILE LYASE IN THE BET V1 SUPERFAMILY Show publication in PURE
2016
Katrin Weinhandl, Melanie Ballach, Margit Winkler, Ahmad Mudassar, Anton Glieder, Ruth Birner-Gruenberger, Ian Fotheringham and Franck Escalettes Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT) Show publication in PURE
Florian Krainer, Barbara Darnhofer, Ruth Birner-Gruenberger and Anton Glieder Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris Show publication in PURE
Lukas Sturmberger, Paal W Wallace, Anton Glieder and Ruth Birner-Gruenberger Synergism of proteomics and mRNA sequencing for enzyme discovery Show publication in PURE
Florian W. Krainer, Michaela A. Gerstmann, Barbara Darnhofer, Ruth Birner-Gruenberger and Anton Glieder Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris Show publication in PURE
Thomas Vogl, Lukas Sturmberger, Thomas Kickenweiz, Richard Wasmayer, Christian Schmid, Anna-Maria Hatzl, Michaela Agnes Gerstmann, Julia Pitzer, Marlies Wagner, Gerhard G Thallinger, Martina Geier and Anton Glieder A Toolbox of Diverse Promoters Related to Methanol Utilization Show publication in PURE
2015
Thomas Vogl, Mudassar Ahmad, Florian Krainer, Helmut Schwab and Anton Glieder Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris Show publication in TUGRAZonline
Elisa Lanfranchi, Eva-Maria Köhler, Barbara Darnhofer, Kerstin Steiner, Ruth Birner-Grünberger, Anton Glieder and Margit Winkler Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection Show publication in TUGRAZonline
Martina Geier, Pia Carina Fauland, Thomas Vogl and Anton Glieder Compact multi-enzyme pathways in P. pastoris Show publication in TUGRAZonline
Florian Krainer and Anton Glieder An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Show publication in TUGRAZonline
Florian Krainer, Simona Capone, Martin Jäger, Thomas Vogl, Michaela Agnes Gerstmann, Anton Glieder, Christoph Herwig and Oliver Spadiut Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris. Show publication in TUGRAZonline
Kerstin Steiner, Anton Glieder and Mandana Gruber Cyanohydrin Formation / Henry ReactionSience of Synthesis1 - 5 Show publication in TUGRAZonline
Florian Krainer, Simona Capone, Martin Jäger, Thomas Vogl, Michaela Agnes Gerstmann, Anton Glieder, Christoph Herwig and Oliver Spadiut Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris. Show publication in PURE
Elisa Lanfranchi, Eva-Maria Köhler, Barbara Darnhofer, Kerstin Steiner, Ruth Birner-Grünberger, Anton Glieder and Margit Winkler Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection Show publication in PURE
Thomas Vogl, Mudassar Ahmad, Florian Krainer, Helmut Schwab and Anton Glieder Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris Show publication in PURE
Florian Krainer and Anton Glieder An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Show publication in PURE
Astrid Weninger, Anton Glieder and Thomas Vogl A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris Show publication in PURE
Martina Geier, Pia Carina Fauland, Thomas Vogl and Anton Glieder Compact multi-enzyme pathways in P. pastoris Show publication in PURE
Kerstin Steiner, Anton Glieder and Mandana Gruber Cyanohydrin Formation / Henry ReactionCyanohydrin Formation / Henry Reaction1-5 Show publication in PURE
2014
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Extracellular transaminases for biocatalysis New BiotechnologyS198 - S198 Show publication in TUGRAZonline
Margit Winkler, Martina Geier, Thorsten Bachler, Steven P Hanlon, Matthias Kittelmann, Anton Glieder, Stephan Lütz and Beat Wirz Human flavin monooxygenase 2: Heterologous expression in E. coli and API modificationNew BiotechnologyS82 - S82 Show publication in TUGRAZonline
Aleksandra Mitrovic and Anton Glieder Discovery of fungal enzymes and pathwaysFungal Bio-Molecules: Sources, Applications and Recent Developments51 - 61 Show publication in TUGRAZonline
Oliver Spadiut, Simona Capone, Florian Krainer, Anton Glieder and Christoph Herwig Microbials for the production of monoclonal antibodies and antibody fragments Show publication in TUGRAZonline
Aleksandra Mitrovic, Karlheinz Flicker, Georg Steinkellner, Karl Gruber, Christoph Reisinger, Christoph Schirrmacher, Andrea Camattari and Anton Glieder Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii Show publication in TUGRAZonline
Barbara Petschacher, Nicole Staunig, Monika Mueller, Martin Schuermann, Daniel Mink, Stefaan Wildemann, Karl Gruber and Anton Glieder Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD (P)+ Regeneration in Biocatalytic Oxidations Show publication in TUGRAZonline
Shuke Wu, Yongzheng Chen, Yi Xu, Anton Glieder and Zhi Li Enantioselective trans-dihydroxylation of aryl olefins by cascade biocatalysis with recombinant escherichia coli coexpressing monooxygenase and epoxide hydrolase Show publication in TUGRAZonline
Andrea Mellitzer, Claudia Ruth, Claes Gustafson, Mark Welch, Ruth Birner-Gruenberger, Roland Weis, Thomas Purkarthoer and Anton Glieder Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks Show publication in TUGRAZonline
Florian Krainer, Robert Pletzenauer, Laura Rossetti, Christoph Herwig, Anton Glieder and Oliver Spadiut Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris. Show publication in TUGRAZonline
Laura Näätsaari, Florian Krainer, Michael Schubert, Anton Glieder and Gerhard Thallinger Peroxidase gene discovery from the horseradish transcriptome Show publication in TUGRAZonline
Thomas Vogl, Gerhard Thallinger, Günther Zellnig, David Drew, James Cregg, Anton Glieder and Maria Elisabeth Freigaßner Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression Show publication in TUGRAZonline
Barbara Petschacher, Nicole Staunig, Monika Müller, Martin Schürmann, Daniel Mink, Stefaan DeWildeman, Karl Gruber and Anton Glieder Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD(P)(+) Regeneration in Biocatalytic Oxidations. Show publication in TUGRAZonline
Diogo Rodrigues, Matthias Kittelmann, Fabian Eggimann, Thorsten Bachler, Sandra Abad, Andrea Camattari, Anton Glieder, Margit Winkler and Stephan Lütz PRODUCTION OF RECOMBINANT HUMAN ALDEHYDE OXIDASE IN E. COLI AND OPTIMIZATION OF ITS APPLICATION FOR PREPARATIVE SYNTHESIS OF OXIDIZED DRUG METABOLITES Show publication in TUGRAZonline
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Carbon source dependent promoters in yeasts Show publication in TUGRAZonline
Laura Näätsaari, Florian Krainer, Michael Schubert, Anton Glieder and Gerhard Thallinger Peroxidase gene discovery from the horseradish transcriptome Show publication in PURE
Oliver Spadiut, Simona Capone, Florian Krainer, Anton Glieder and Christoph Herwig Microbials for the production of monoclonal antibodies and antibody fragments Show publication in PURE
Margit Winkler, Martina Geier, Thorsten Bachler, Steven P Hanlon, Matthias Kittelmann, Anton Glieder, Stephan Lütz and Beat Wirz Human flavin monooxygenase 2: Heterologous expression in E. coli and API modificationHuman flavin monooxygenase 2: Heterologous expression in E. coli and API modificationS82-S82 Show publication in PURE
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Extracellular transaminases for biocatalysisExtracellular transaminases for biocatalysisS198-S198 Show publication in PURE
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari Carbon source dependent promoters in yeasts Show publication in PURE
Barbara Petschacher, Nicole Staunig, Monika Mueller, Martin Schuermann, Daniel Mink, Stefaan Wildemann, Karl Gruber and Anton Glieder Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD (P)+ Regeneration in Biocatalytic Oxidations Show publication in PURE
Florian Krainer, Robert Pletzenauer, Laura Rossetti, Christoph Herwig, Anton Glieder and Oliver Spadiut Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris. Show publication in PURE
Andrea Mellitzer, Claudia Ruth, Claes Gustafson, Mark Welch, Ruth Birner-Gruenberger, Roland Weis, Thomas Purkarthoer and Anton Glieder Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks Show publication in PURE
Aleksandra Mitrovic and Anton Glieder Discovery of fungal enzymes and pathwaysDiscovery of fungal enzymes and pathways51-61 Show publication in PURE
Shuke Wu, Yongzheng Chen, Yi Xu, Anton Glieder and Zhi Li Enantioselective trans-dihydroxylation of aryl olefins by cascade biocatalysis with recombinant escherichia coli coexpressing monooxygenase and epoxide hydrolase Show publication in PURE
Diogo Rodrigues, Matthias Kittelmann, Fabian Eggimann, Thorsten Bachler, Sandra Abad, Andrea Camattari, Anton Glieder, Margit Winkler and Stephan Lütz PRODUCTION OF RECOMBINANT HUMAN ALDEHYDE OXIDASE IN E. COLI AND OPTIMIZATION OF ITS APPLICATION FOR PREPARATIVE SYNTHESIS OF OXIDIZED DRUG METABOLITES Show publication in PURE
Thomas Vogl, Gerhard Thallinger, Günther Zellnig, David Drew, James Cregg, Anton Glieder and Maria Elisabeth Freigaßner Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression Show publication in PURE
Aleksandra Mitrovic, Karlheinz Flicker, Georg Steinkellner, Karl Gruber, Christoph Reisinger, Christoph Schirrmacher, Andrea Camattari and Anton Glieder Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii Show publication in PURE
2013
Martina Geier, Christian Schmid and Anton Glieder First functional expression of cytochrome P450 3A4 in Pichia pastoris Show publication in TUGRAZonline
Martina Geier, Andreas Braun, Patrick Fladischer, Piotr Stepniak, Florian Rudroff , Christoph Hametner , Marko Mihovilovic and Anton Glieder Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation Show publication in TUGRAZonline
Florian Krainer, Christoph Gmeiner, Lukas Neutsch, Markus Windwarder, Robert Pletzenauer, Christoph Herwig, Friedrich Altmann, Anton Glieder and Oliver Spadiut Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris Show publication in TUGRAZonline
Thomas Vogl, Claudia Ruth, Julia Pitzer, Thomas Kickenweiz and Anton Glieder Synthetic Core Promoters for Pichia pastoris Show publication in TUGRAZonline
Elisa Lanfranchi, Kerstin Steiner, Anton Glieder, Ivan Hajnal, Roger A. Sheldon, Sander van Pelt and Margit Winkler RECENT DEVELOPMENTS IN HYDROXYNITRILE LYASES FOR INDUSTRIAL BIOTECHNOLOGY Show publication in TUGRAZonline
Florian Krainer, Christoph Gmeiner, Lukas Neutsch, Markus Windwarder, Robert Pletzenauer, Christoph Herwig, Friedrich Altmann, Anton Glieder and Oliver Spadiut Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris Show publication in PURE
Martina Geier, Andreas Braun, Patrick Fladischer, Piotr Stepniak, Florian Rudroff, Christoph Hametner, Marko Mihovilovic and Anton Glieder Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation Show publication in PURE
Martina Geier, Christian Schmid and Anton Glieder First functional expression of cytochrome P450 3A4 in Pichia pastoris Show publication in PURE
Elisa Lanfranchi, Kerstin Steiner, Anton Glieder, Ivan Hajnal, Roger A. Sheldon, Sander van Pelt and Margit Winkler RECENT DEVELOPMENTS IN HYDROXYNITRILE LYASES FOR INDUSTRIAL BIOTECHNOLOGY Show publication in PURE
Thomas Vogl, Claudia Ruth, Julia Pitzer, Thomas Kickenweiz and Anton Glieder Synthetic Core Promoters for Pichia pastoris Show publication in PURE
2012
Florian Krainer, Christian Dietzsch, Tanja Hajek, Christoph Herwig, Oliver Spadiut and Anton Glieder Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway Show publication in TUGRAZonline
Christoph Sygmund, Alexander Gutmann, Iris Krondorfer, Magdalena Kujawa, Anton Glieder, Beate Pscheidt, Dietmar Haltrich, Clemens Peterbauer and Roman Kittl Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris Show publication in TUGRAZonline
Andreas Braun, Martina Geier, Bruno Buehler, Andreas Schmid, Stephan Mauersberger and Anton Glieder Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes Show publication in TUGRAZonline
Martina Geier, Andreas Braun, Anita Emmerstorfer, Harald Pichler and Anton Glieder Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study Show publication in TUGRAZonline
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari A NOVEL MULTI-ENZYMATIC ASSAY FOR TRANSAMINASE ACTIVITY: HIGH THROUGHPUT SCREENING APPLICATIONS IN PICHIA PASTORIS Show publication in TUGRAZonline
Birgit Wilding, Margit Winkler, Regina Kratzer, Barbara Petschacher, Anton Glieder and Norbert Klempier NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION Show publication in TUGRAZonline
Andreas Braun, Bettina Halwachs, Martina Geier, Katrin Weinhandl, Michael Guggemos, Jan Marienhagen, Anna Joel Ruff, Ulrich Schwaneberg, Daniel E. Torres Pazmiño, Vincent Rabin, Gerhard Thallinger and Anton Glieder MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes Show publication in TUGRAZonline
Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz and Margit Winkler EXPRESSION OF RECOMBINANT HUMAN FLAVIN MONOOXYGENASE AND MOCLOBEMIDE N-OXIDE SYNTHESIS ON MULTI-MG SCALE Show publication in TUGRAZonline
Margit Winkler, Anton Glieder and Kerstin Steiner C–X Bond Formation: Hydroxynitrile lyases: From Nature to ApplicationComprehensive Chirality350 - 371 Show publication in TUGRAZonline
Andreas Braun, Bettina Halwachs, Martina Geier, Katrin Weinhandl, Michael Guggemos, Jan Marienhagen, Anna Joel Ruff, Ulrich Schwaneberg, Daniel E. Torres Pazmiño, Vincent Rabin, Gerhard Thallinger and Anton Glieder MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes Show publication in PURE
Florian Krainer, Christian Dietzsch, Tanja Hajek, Christoph Herwig, Oliver Spadiut and Anton Glieder Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway Show publication in PURE
Christoph Sygmund, Alexander Gutmann, Iris Krondorfer, Magdalena Kujawa, Anton Glieder, Beate Pscheidt, Dietmar Haltrich, Clemens Peterbauer and Roman Kittl Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris Show publication in PURE
Martina Geier, Andreas Braun, Anita Emmerstorfer, Harald Pichler and Anton Glieder Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study Show publication in PURE
Katrin Weinhandl, Margit Winkler, Anton Glieder and Andrea Camattari A NOVEL MULTI-ENZYMATIC ASSAY FOR TRANSAMINASE ACTIVITY: HIGH THROUGHPUT SCREENING APPLICATIONS IN PICHIA PASTORIS Show publication in PURE
Birgit Wilding, Margit Winkler, Regina Kratzer, Barbara Petschacher, Anton Glieder and Norbert Klempier NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION Show publication in PURE
Margit Winkler, Anton Glieder and Kerstin Steiner C–X Bond Formation: Hydroxynitrile lyases: From Nature to ApplicationC–X Bond Formation: Hydroxynitrile lyases: From Nature to Application350-371 Show publication in PURE
Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz and Margit Winkler Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale Show publication in PURE
Andreas Braun, Martina Geier, Bruno Buehler, Andreas Schmid, Stephan Mauersberger and Anton Glieder Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes Show publication in PURE
Andrea Mellitzer, Anton Glieder, Roland Weis, Christoph Reisinger and Karlheinz Flicker Sensitive high-throughput screening for the detection of reducing sugars Show publication in PURE
Andrea Mellitzer, Roland Weis, Anton Glieder and Karlheinz Flicker Expression of lignocellulolytic enzymes in Pichia pastoris Show publication in PURE
Laura Näätsaari, Beate Mistlberger, Claudia Ruth, Tanja Hajek, Franz Stefan Hartner and Anton Glieder Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology Show publication in PURE
2011
Andrea Hasmann, Anita Eberl, Konstantin Schneider, K.-U. Schoening, F. Kaufmann, Anton Glieder, J. Kovac, Artur Cavaco-Paulo, Eva Wehrschütz-Sigl and Georg Gübitz Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3 Show publication in TUGRAZonline
Andreas Küberl, Jessica Schneider, Gerhard Thallinger, Ingund Anderl, Daniel Wibberg, Tanja Hajek, Sebastian Jaenicke, Karina Brinkrolf, Alexander Goesmann, Rafael Szczepanowski, Alfred Pühler, Helmut Schwab, Anton Glieder and Harald Pichler High-quality genome sequence of Pichia pastoris CBS7435 Show publication in TUGRAZonline
Sandra Abad, Jozef Nahalka, Margit Winkler, Gabriele Bergler, Robert Speight, Anton Glieder and Bernd Nidetzky HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS Show publication in TUGRAZonline
Matthias Schittmayer, Anton Glieder, Michael Karl Uhl, Andreas Winkler, Simone Zach, Jörg Schrittwieser, Wolfgang Kroutil, Peter Macheroux, Karl Gruber, Spiros Kambourakis, J. David Rozzell and Margit Winkler Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones Show publication in TUGRAZonline
Andreas Küberl, Jessica Schneider, Gerhard Thallinger, Ingund Anderl, Daniel Wibberg, Tanja Hajek, Sebastian Jaenicke, Karina Brinkrolf, Alexander Goesmann, Rafael Szczepanowski, Alfred Pühler, Helmut Schwab, Anton Glieder and Harald Pichler High-quality genome sequence of Pichia pastoris CBS7435 Show publication in PURE
Sandra Abad, Jozef Nahalka, Margit Winkler, Gabriele Bergler, Robert Speight, Anton Glieder and Bernd Nidetzky HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS Show publication in PURE
Andrea Hasmann, Anita Eberl, Konstantin Schneider, K.-U. Schoening, F. Kaufmann, Anton Glieder, J. Kovac, Artur Cavaco-Paulo, Eva Wehrschütz-Sigl and Georg Gübitz Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3 Show publication in PURE
Matthias Schittmayer, Anton Glieder, Michael Karl Uhl, Andreas Winkler, Simone Zach, Jörg Schrittwieser, Wolfgang Kroutil, Peter Macheroux, Karl Gruber, Spiros Kambourakis, J. David Rozzell and Margit Winkler Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones Show publication in PURE
2010
Bernd Nidetzky, Sandra Abad, Jozef Nahalka, Robert Speight, I. Fotheringhan and Anton Glieder Stepwise engineering of a Pichia pastoris D-amino acid whole cell catalyst Show publication in TUGRAZonline
Ulrike Schreiner, Bianca Hecher, Sascha Obrowsky, Kerstin Waich, Norbert Klempier, Georg Steinkellner , Karl Gruber, J. David Rozzell, Anton Glieder and Margit Winkler Directed evolution of Alcaligenes faecalis nitrilase Show publication in TUGRAZonline
Claudia Ruth and Anton Glieder Perspectives on synthetic promoters for biocatalysis and biotransformation Show publication in TUGRAZonline
Sandra Abad, Kerstin Kitz, Astrid Hörmann, Ulrike Schreiner, Franz Stefan Hartner and Anton Glieder Real time PCR based determination of gene copy numbers in Pichia pastoris Show publication in TUGRAZonline
Daniel E Torres Pazmiño, Margit Winkler, Anton Glieder and Marco W Fraaije Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications Show publication in TUGRAZonline
Kirsten Schroer, Klaus Peter Luef, Franz Stefan Hartner, Anton Glieder and Beate Pscheidt Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation Show publication in TUGRAZonline
Ulrike Schreiner, Bianca Hecher, Sascha Obrowsky, Kerstin Waich, Norbert Klempier, Georg Steinkellner, Karl Gruber, J. David Rozzell, Anton Glieder and Margit Winkler Directed evolution of Alcaligenes faecalis nitrilase Show publication in PURE
Sandra Abad, Kerstin Kitz, Astrid Hörmann, Ulrike Schreiner, Franz Stefan Hartner and Anton Glieder Real time PCR based determination of gene copy numbers in Pichia pastoris Show publication in PURE
Kirsten Schroer, Klaus Peter Luef, Franz Stefan Hartner, Anton Glieder and Beate Pscheidt Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation Show publication in PURE
Daniel E Torres Pazmiño, Margit Winkler, Anton Glieder and Marco W Fraaije Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications Show publication in PURE
Claudia Ruth and Anton Glieder Perspectives on synthetic promoters for biocatalysis and biotransformation Show publication in PURE
Bernd Nidetzky, Sandra Abad, Jozef Nahalka, Robert Speight, I. Fotheringhan and Anton Glieder Stepwise engineering of a Pichia pastoris D-amino acid whole cell catalyst Show publication in PURE

Team Leader
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Anton Glieder
Ao.Univ.-Prof. Mag.rer.nat. Dr.rer.nat.