Computational Bioprocess Engineering

Enabling realistic simulations of complex industrial scale devices to be used in engineering processes is our goal. We find, test, validate and interconnect highly efficient algorithms for graphic processing units (GPUs) to simulate the physical process inside devices like the bioreactor.

Our in-house code base allows us to create customized applications that include the movement of the fluid flow field, bubbles, particles, droplets, species and microorganisms as well as the device geometry including stirrers, heat exchangers, porous zones and sensors. The biological activity of the microorganisms can be included using metabolic models.

F.l.t.r.: Simon Rustige, Philipp Eibl, Oliver Tomaschitz,
Gottfried Segner, Laura Schneider, Christian Witz

Aerated and stirred 150l bioreactor
with three Rushton turbines

In his PhD Christian Witz rewrote and adapted a preexisting CPU programm to be used on GPUs (Paper 1) and achieved a huge speedup using newly developed Lattice-Boltzmann algorithms (LBM), Lagrangian particle tracking (LPT) and efficient parallelisation strategies. The simulations were validated using a 150l acrylic glas pilot plant reactor and a conductivity based local holdup measurement system. Finally simulations of industrial sized reactors up to 250 m³ were possible (Paper 2).

 

Since then the functionalities of the program have been constantly expanded. Non-Newtonian fluids can be simulated and up to 32 GPUs can be used to increase the simulation speed. The power consumption of the stirrer is determined during the simulation as well as the shear rate distribution in the reactor. Species like nutrition, dissolved oxygen or carbon dioxide are transported by the fluid flow field and the path of the microorganisms in the reactor (lifelines) can be followed.

Distribution of species in a stirred tank with a tilted stirrer axis

In his PhD Philipp Eibl is working on additional physical models in order to analyse the full biological process in-silico. This includes simulation of the temperature distribution inside the reactor with heat sources such as active organisms as well as heat sinks such as heat exchangers or cooling jackets. Nutrients are not only present as dissolved substrate but may also be present as a dispersed immiscible phase that can be modelled with highly resoluted Eulerian methods or Population Balance Models, depending on the droplet size.

 

Ultimately, complex biological models are coupled with the fluid flow field. This not only includes prediction of growth and production rate of funghi or cell cultures, but also mechanical influences on cell damage or fungal morphology and size distribution. All these models are implemented with special attention to computational efficiency, using state-of-the-art GPU-Technology.

Team

Contact
Christian Witz
Dipl.-Ing. Dr.techn. BSc

Institute of Process and Particle Engineering
Inffeldgasse 13/III
8010 Graz

Phone
+43 (316) 873 - 30416
Fax
+43 (316) 873 - 1030416
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