2016
Norbert Klempier Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli The journal of biological chemistry 291, 25411-25426, 2016
2015
Birgit Wilding, Stefan Faschauner and Norbert Klempier A practical synthesis of 5-functionalized thieno[2,3-d]pyrimidines Tetrahedron letters 56, 4486-4489, 2015
Birgit Wilding, Carina Vidovic and Norbert Klempier A convenient synthetic route to substituted pyrrolo[2,3-b]pyridines via a novel ethylene-bridged compound Tetrahedron letters 56, 6606-6609, 2015
Birgit Wilding, Alicja B Vesela, Justin J. B. Perry, Gary W. Black, Meng Zhang, Ludmila Martinkova and Norbert Klempier An investigation of nitrile transforming enzymes in the chemo-enzymatic synthesis of the taxol sidechain Organic & biomolecular chemistry 13, 7803-7812, 2015
2014
Margit Winkler, Kamilla Napora-Wijata, Birgit Wilding and Norbert Klempier Enzymatic Reductions of unconventioal substrates: Carboxylates and Nitriles
Margit Winkler, Kamilla Napora-Wijata, Birgit Wilding and Norbert Klempier Unconventional Enzymatic Reductions: Nitriles and Carboxylates as Substrates
Norbert Klempier ACIB Jahresbericht 2014 Enzymatic Nitrile Reduction
Katharina Dokulil, Birgit Wilding, Gerlinde Offenmüller, Norbert Klempier and Margit Winkler In Vitro Reconstruction of Queuosine biosynthesis in Geobacillus kaustophilus: two step cascade from 7-carboxy-7-deazaguanin to PreQ1
Margit Winkler, Kamilla Napora-Wijata, Birgit Wilding and Norbert Klempier Unconventional Enzymatic Reductions: Nitriles and Carboxylates as SubstratesNew BiotechnologyS1-S2
2013
Norbert Klempier ACIB Jahresbericht 2013 Enzymatic Nitrile Reduction
Stefan Faschauner, Wilfried Sailer-Kronlachner, Melanie Zechner, Birgit Wilding and Norbert Klempier Syntheses of substituted thieno-, cyclopenta- and pyrazolopyrimidines
Birgit Wilding, Alicja Veselá, Jasmin Nathalie Resch, Ludmila Martínková and Norbert Klempier Biocatalytic synthesis of taxol sidechain precursors
Birgit Wilding, Barbara Petschacher, Regina Kratzer, Georg Steinkellner, Andrzej Franciszek Lyskowski and Norbert Klempier Exploring the active binding site of nitrile reductase queF
Birgit Wilding, Barbara Petschacher, Margit Winkler, Gerlinde Offenmüller, Regina Kratzer, Georg Steinkellner, Andrzej Franciszek Lyskowski, Karl Gruber, Bernd Nidetzky and Norbert Klempier Exploring the Active Binding Site of Nitrile Reductases
Birgit Wilding, Barbara Petschacher, Margit Winkler, Regina Kratzer, Georg Steinkellner, Andrzej Franciszek Lyskowski, Karl Gruber, Bernd Nidetzky and Norbert Klempier Biological nitrile reduction – exploring a remarkable reaction
Norbert Klempier ACIB International Scientific Advisory Board - Project Report Enzymatic Nitrile Reduction
Birgit Wilding, Barbara Petschacher, Margit Winkler, Gerlinde Offenmüller, Georg Steinkellner, Andrzej Franciszek Lyskowski, Regina Kratzer, Karl Gruber, Bernd Nidetzky and Norbert Klempier Enzymatic Nitrile Reduction
Birgit Wilding, Margit Winkler, Barbara Petschacher, Regina Kratzer, Sigrid Egger, Georg Steinkellner, Andrzej Franciszek Lyskowski, Bernd Nidetzky, Karl Gruber and Norbert Klempier Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering Chemistry - a European journal 19, 7007-7012, 2013
Birgit Wilding, Alicja Veselá, Jasmin Resch, Justin Perry, Gary Black, Meng Zhang, Ludmila Martínková and Norbert Klempier Synthesis of a taxol sidechain precursor with bacterial and fungal nitrilases
2012
Birgit Wilding, Maria Koshanskaya, Margit Winkler, Justin Perry, Gary Black and Norbert Klempier A Two Enzyme Approach to a Taxol Sidechain Precursor
Birgit Wilding, Margit Winkler, Regina Kratzer, Gerlinde Offenmüller, Georg Steinkellner, Andrzej Franciszek Lyskowski, Karl Gruber, Bernd Nidetzky and Norbert Klempier Enzymatic Nitrile Reduction
Birgit Wilding, Margit Winkler, Regina Kratzer, Barbara Petschacher, Anton Glieder and Norbert Klempier NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION Advanced Synthesis & Catalysis 354, 2191-2198, 2012
Norbert Klempier ACIB International Scientific Advisory Board - Project Report Enzymatic Nitrile Reduction
Birgit Wilding, Margit Winkler, Regina Kratzer, Margaretha Schiller, Gerlinde Offenmüller, Sigrid Egger, Georg Steinkellner, Andrzej Franciszek Lyskowski, Karl Gruber, Bernd Nidetzky and Norbert Klempier Enzymatic Nitrile Reduction
Norbert Klempier ACIB Jahresbericht 2012 Enzymatic Nitrile Reduction
Birgit Wilding, Margit Winkler, Regina Kratzer, Barbara Petschacher, Sigrid Egger, Georg Steinkellner, Andrzej Franciszek Lyskowski, Gerlinde Offenmüller, Margaretha Schiller, Bernd Nidetzky, Karl Gruber and Norbert Klempier Investigations of the substrate scope of nitrile reductase queF
Birgit Wilding, Cornelia Hojnik, Margit Winkler and Norbert Klempier Synthesis of 7-deazaguanine analogues including a novel biotransformation step
Norbert Klempier, Birgit Wilding, Margit Winkler, Regina Kratzer, Sigrid Egger, Georg Steinkellner, Andrzej Lyskowski, Bernd Nidetzky and Karl Gruber Biocatalytic nitrile reduction with wild type and mutant nitrile reductases
2011
Birgit Wilding, Barbara Petschacher, Margit Winkler, Sigrid Egger, Regina Kratzer, Margaretha Schiller, Bernd Nidetzky, Georg Steinkellner, Andrzej Lyskowski, Karl Gruber and Norbert Klempier Enzymatic Nitrile Reduction
Carina Hasenöhrl, Birgit Wilding, Gary Black, Justin Perry, Margit Winkler and Norbert Klempier Biocatalytic synthesis of an enantioenriched taxol sidechain precursor
Birgit Wilding, Carina Hasenöhrl, Gary Black, Justin Perry, Margit Winkler and Norbert Klempier PREPARATION OF AN ENANTIOENRICHED TAXOL SIDECHAIN PRECURSOR BY NITRILASE AND NITRILE HYDRATASE
Birgit Wilding and Norbert Klempier Enzymatic Nitrile Reduction
Norbert Klempier Enzymatic Nitrile reduction
Birgit Wilding, Margit Winkler, Barbara Petschacher, Regina Kratzer, Margaretha Schiller, Sigrid Egger, Bernd Nidetzky, Georg Steinkellner, Andrzej Franciszek Lyskowski, Karl Gruber and Norbert Klempier Nitrile Reduction using Nitrile Reductase
Birgit Wilding, Norbert Klempier, Barbara Petschacher, Margit Winkler, Sigrid Egger, Regina Kratzer, Bernd Nidetzky, Georg Steinkellner, Andrzej Lyskowski and Karl Gruber Enzymatic Nitrile Reduction using wild type and mutant Nitrile Reductase QueF
Birgit Wilding, Norbert Klempier, Barbara Petschacher, Margit Winkler, Sigrid Egger, Regina Kratzer, Bernd Nidetzky, Georg Steinkellner, Andrzej Lyskowski and Karl Gruber Enzymatic Nitrile Reduction using wild type and mutant Nitrile Reductase QueFBook of Abstracts76-76
Birgit Wilding, Carina Hasenöhrl, Gary Black, Margit Winkler, Justin Perry and Norbert Klempier Preparation of an enantioenriched taxol side chain precursor by nitrilase and nitrile hydrataseBook of Abstracts76-76
2010
Norbert Klempier, Birgit Wilding and Margit Winkler Biotransformation of Purine and Pyrimidine Carbonitriles
Birgit Wilding and Norbert Klempier Biotransformation of Purine and Pyrimidine CarbonitrilesBook of abstracts150-150
Ulrike Schreiner, Bianca Hecher, Sascha Obrowsky, Kerstin Waich, Norbert Klempier, Georg Steinkellner, Karl Gruber, J. David Rozzell, Anton Glieder and Margit Winkler Directed evolution of Alcaligenes faecalis nitrilase Enzyme and microbial technology 47, 140-146, 2010
Ulrike Schreiner, Norbert Klempier, J. David Rozzell, Anton Glieder and Margit Winkler Engineered Alcaligenes faecalis nitrilases
Norbert Klempier ACIB Jahresbericht 2010
Birgit Wilding and Norbert Klempier Biotransformation of Purine, Pyrimidine and Pyrrole Carbonitriles
2009
Margit Winkler, Dorith Meischler, Astrid-C. Knall, Ulrike Schreiner, Martin R. Kulterer, Stefan Koller, Gabriele Kremser, Christoph Auner, Olivia Schnitzer, Martin Schmuck, Barbara Enko, Anita Leitgeb, Bastian Harum, Johann Hlina, Ulrike Richter, Benjamin Stickler and Norbert Klempier Nitrile transforming enzymes in enantioselective synthesis of non-proteinogenic amino acids
Norbert Klempier and Margit Winkler Nitrilase and Nitrile Hydratase Catalyzed Enantioselective Preparation of Non-proteinogenic Amino AcidsModern Biocatalysis247-260
Margit Winkler and Norbert Klempier ENANTIOSEPARATION OF NONPROTEINOGENIC AMINO ACIDS Analytical and bioanalytical chemistry 393, 1789-1796, 2009
Norbert Klempier and Margit Winkler Enantioseparation of Non-proteinogenic Amino Acids Analytical and bioanalytical chemistry 393, 1789-1796, 2009
Margit Winkler, Ondrej Kaplan, Vojtech Vejvoda, Norbert Klempier and Ludmila Martinkova BIOCATALYTIC APPLICATION OF NITRILASES FROM FUSARIUM SOLANI O1 AND ASPERGILLUS NIGER K10 Journal of molecular catalysis / B 59, 243-247, 2009
2008
Margit Winkler, Norbert Klempier, Hansjörg Weber, Katrin R. Grünwald, Michaela Flock and Alk Dransfeld Influence of relative configuration of disubstituted cyclopentanes and –hexanes on 13C shifts Magnetic resonance in chemistry 46, 865-871, 2008
2007
Margit Winkler, Astrid-Caroline Knall, Martin R. Kulterer and Norbert Klempier Nitrilases catalyze Key Step to conformationally constrained GABA analogous gamma-Amino Acids in high Optical Purity The journal of organic chemistry 72, 7423-7426, 2007
Margit Winkler, Martin R. Kulterer, Benjamin Stickler and Norbert Klempier Nitrilase catalyzes key step to gamma-amino acids in high optical purity
Margit Winkler and Norbert Klempier Asymmetric Synthesis of Cyclic Amino Acids
Margit Winkler, Dorith Meischler and Norbert Klempier Nitrilase catalyzed enantioselective Synthesis of Pyrrolidine and Piperidine Carboxylic Acids Advanced Synthesis & Catalysis 349, 1475-1480, 2007
Margit Winkler, Dorith Meischler, Hansjörg Weber, Johann Hlina, Harald Damej and Norbert Klempier Nitrilase catalyzed enantioselective Synthesis of Pyrrolidine and Piperidine Carboxylic Acids
2006
Margit Winkler, Astrid-Caroline Knall, Dorith Meischler, Stefan Koller, Anita Leitgeb and Norbert Klempier Nitrilase catalyzed Synthesis of GABA Transition State Analogues
Katrin Renate Grünwald, Margit Winkler and Norbert Klempier SYNTHESIS OF DIASTEREOISOMERIC ß- AND y-FLUORO NITRILES
Norbert Klempier Biotransformations employing Nitrile converting Enzymes
Margit Winkler, Anton Glieder and Norbert Klempier Enzyme stabilizer DTT catalyzes nitrilase analogue hydrolysis of nitriles Chemical communications , 1298-1300, 2006
Norbert Klempier and Margit Winkler Nitrilase catalyzed Synthesis of GABA Transition State Analogues
2005
Astrid-Caroline Knall, Margit Winkler and Norbert Klempier Synthese regio- und stereoisomerer ß-Aminoindannitrile
Margit Winkler, Martin Schmuck and Norbert Klempier Enantioselective microbial transformation of ß-amino nitriles
Margit Winkler, Sieglinde Ebner and Norbert Klempier Enzyme Mediated Three Step Preparation of the TAXOL-side Chain Starting from Benzaldehyde.
Margit Winkler, Martin Schmuck and Norbert Klempier Enantioselective microbial transformation of ß-amino nitrilesBiotrans 2005 Symposium, The key to Industrial Biotechnology1-349
Astrid-Caroline Knall, Margit Winkler, Kurt Stubenrauch and Norbert Klempier Microbial preparation of Cispentacin-derivativesBiotrans 2005 Symposium - The Key to Industrial Biotechnology188-188
Margit Winkler, Siglinde Ebner and Norbert Klempier Enzyme mediated three step Preparation of the Taxol SideChain starting from Benzaldehyde
Norbert Klempier, Margit Winkler and Astrid-Caroline Knall Enantioselective preparation of ß-amino acids and amides using nitrile hydrolyzing enzymes
Astrid C. Knall, Margit Winkler and Norbert Klempier Microbial preparation of Cispentacin-derivatives
2004
Astrid-Caroline Knall, Margit Winkler and Norbert Klempier Synthese regio- und stereoisomerer ß-Indannitrile
Margit Winkler and Norbert Klempier Trends of nitrile hydratase/amidase catalyzed transformation of alicyclic ß-amino nitrilesbook of abstracts, biocat 2004, Second International Congress on Biocatalysis1-278
Margit Winkler and Norbert Klempier Trends of nitrile hydratase/amidase catalyzed transformation of alicyclic ß-amino nitriles
Margit Winkler, Norbert Klempier, Ludmila Martinkova and Herfried Griengl Microbial Hydrolysis of ß-Aminonitriles
Margit Winkler, Helmut Hönig and Norbert Klempier Biotransformation of beta-amino nitriles: the role of the N-protecting group Journal of molecular catalysis / B , 115-121, 2004
Margit Winkler and Norbert Klempier Trends of nitrile hydratase / amidase catalyzed transformation of alicyclic -amino nitriles
2003
Helmut Schwab, Norbert Klempier and Michaela Pressnig Biocatalytic Characterization of Esterases Journal of biotechnology , 2003
Margit Preiml, Kerstin Hillmayer and Norbert Klempier Biotransformation of N-protected b-Amino Nitriles to b-Amino AcidsChemické Listy 97,6325-526
Margit Preiml, Kerstin Hillmayer and Norbert Klempier A NEW APPROACH TO ß-AMINO ACIDS: BIOTRANSFORMATION OF N-PROTECTED ß-AMINO NITRILES Tetrahedron letters 44, 5057-5059, 2003
Margit Winkler, Kerstin Hillmayer and Norbert Klempier Biotransformation of N-protected ß-Amino Nitriles to ß-Amino Acids.
2002
Ludmila Martinkova, Norbert Klempier, Margit Preiml, Maria Ovesna, Marek Kuzma, Veronika Mylerova and Vladimir Kren SELECTIVE BIOTRANSFORMATION OF SUBSTITUTED ALICYCLIC NITRILES BY RHODOCOCCUS EQUI A4 Canadian journal of chemistry 80, 724-727, 2002
2001
L. Martinková, Norbert Klempier, J. Badrdakji, A. Kandelbauer, M. Ovesná, T. Podarilová, M. Kuzma, I. Prepechalová, Herfried Griengl and V. Kren Biotransformation of 3-substituted methyl (R,S)-4-cyanobutanoates with nitrile-and amide-converting biocatalysts. Journal of molecular catalysis / B , 95-99, 2001
Ludmila Martinkova and Norbert Klempier Biocatalytic potential of a nitrile converting microorganism Rhodococcus equi strain A4
Ludmila Martinkova, Maria Ovesna, Norbert Klempier, Margit Preiml, D. Crestia, C. Demuynck, J. Bolte, R. Liz, A. M. Testera, V. Gotor, I. Prepechalova, Marek Kuzma, V. Prikrylova, Veronika Mylerova and Vladimir Kren ASYMMETRIC TRANSFORMATIONS BY COUPLED ENZYME AND METAL BIOCATALYTIC POTENTIAL OF A NITRILE-CONVERTING MICROORGANISM, RHODOCOCCUS EQUI STRAIN A4BIOTRANS 20011-492
2000
Michaela Pressnig, Norbert Klempier, Herfried Griengl and Helmut Schwab Biocatalytic characteristics of bacterial esterases
1999
L. Martinková, Norbert Klempier, I. Prepechalová, V. Prikrylová, M. Ovesná, Herfried Griengl and V. Kren Proton-NMR-Analyses of the Substrate Specificity of a ß-Ketolase from Pseudomonas putida, Acetopyruvate Hydrolase Biotechnology letters 20, 909-912, 1999
Michaela Pressnig, Norbert Klempier, Herfried Griengl and Helmut Schwab Biocatalytic characteristics of bacterial
Maria Ovesna, Ludmila Martinkova and Norbert Klempier Biotransformations of 2-hydroxynitriles and 3-hydroxynitriles by Rhodococcus equi strain A4
Michael Tauber, Norbert Klempier and Georg Gübitz Nitrile hydratase and amidase from Rhodococcus rhodochrous Hydrolysis of acrylic fibres and granulates
Norbert Klempier Chemoselective Biotransformation of Nitriles by Rhodococcus equi A4. Biotechnology letters 10, 909-912, 1999
1998
Herfried Griengl, Norbert Klempier, P. Pöchlauer, Markus Schmidt, N. Shi and A. Mackova Enzyme Catalysed Formation of (S)-Cyanohydrins Derived from Aldehydes and Ketones in a Biphasic Solvent System Tetrahedron 54, 14477-14486, 1998
1997
A. Schlacher, T. Stanzer, B. Soelkner, E. Klingsbichel, E. Petersen, M. Schmidt, Norbert Klempier and Helmut Schwab New Pseudomonas esterases by genetic engineering Journal of molecular catalysis / B 3, 25-27, 1997
Ludmila Martinkova, Irena Prepechalova, Norbert Klempier, Vladimir Kren and Herfried Griengl Chemoselective Biotransformations of nitriles by Rhodococcus equi strain A4
1996
Norbert Klempier Chemoselective Hydrolysis of Nitriles by Rhodococcus rhodochrous NCIMB 11216 Food technology and biotechnology 34, 67-70, 1996
A. Schlacher, T. Stanzer, E. Klingsbichel, E. Petersen, B. Soelkner, I. Osprian, M. Schmidt, Norbert Klempier, Kurt Faber and Helmut Schwab Recombinant Esterases for Organic Synthesis Mededelingen / Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent 61, 1391-1394, 1996
1995
Michael Schmidt, Stephanie Herve, Norbert Klempier and Herfried Griengl Hevea brasiliensis hydroxynitrile lyase: substrates and selectivities
Norbert Klempier, Ulrike Pichler and Herfried Griengl Synthesis of alpha,-beta-unsaturated (S)-Cyanohydrins using the Hydroxynitrile Lyase from Hevea brasiliensis Tetrahedron / Asymmetry 6, 845-848, 1995
Norbert Klempier, Helmut Schwab and et al. New esterases for organic synthesis by genetic engeneering
Norbert Klempier, Helmut Schwab and et al. Recombinant esterases for organic synthesis
Anton Schlacher, Norbert Klempier, Helmut Schwab and et al. Recombinant esterases for organic synthesis
1994
Elisabeth Klingsbichel, Norbert Klempier, Helmut Schwab and et al. New esterases for organic synthesis by genetic engeneering
Elisabeth Klingsbichel, Norbert Klempier, Helmut Schwab and et al. Molecular cloning and overexpression of an esterase gene from Pseudomonas marginata
Norbert Klempier, Nongjan Shi and Herfried Griengl Lyase catalyzed preparation of enantiomerically enriched cyanohydrins of aliphatic ketones
1993
Norbert Klempier, Herfried Griengl and Marianne Hayn Aliphatic (S)-cyanohydrins by enzyme catalyzed synthesis Tetrahedron letters 34, 4769-4772, 1993
Michael Schall, Marianne Hayn, Norbert Klempier and Hermann Esterbauer Purification of a hydroxynitrile lyase from Hevea brasiliensis
Anna De Raadt, Herfried Griengl, Norbert Klempier and Arnold Stütz A mild and simple enzymatic conversion of aldono and aldurononitriles into the corresponding amides and/or carboxylic acids The journal of organic chemistry 58, 3179-3184, 1993
Norbert Klempier Asymmetric Hydrolysis of Epoxides using an immobilised Enzyme Preparation from Rhodococcus sp. Tetrahedron 4, 1161-1164, 1993
Norbert Klempier, Ulrike Pichler and Herfried Griengl Enzyme catalyzed synthesis of bifunctional aliphatic (S)-cyanohydrins
Norbert Klempier, Ulrike Pichler, Herfried Griengl, Marianne Hayn and Walter Steiner Aliphatic (S)-cyanohydrins by a hydroxynitrile lyase from Hevea brasiliensis