Winkler Team


Research Goals

Margit Winkler is fascinated by the power of natural biocatalysts for chemical synthesis. Many of the reactions that one may want to use for the preparation of organic molecules have not yet been developed, or have some limitations such as: the need for highly complicated and expensive catalysts, multiple steps or they simply suffer from low selectivity.

The overall theme of Margit's research is to find new and alternative ways to catalyse a transformation by using enzymes. Sometimes, the goal is to determine the scope and limitations of an enzyme and possibly its engineering towards desired characteristics to overcome these limitations. Other times, it is the discovery and detailed study of enzymes that are not well known yet, in order to understand them better and to determine whether and how they could become applicable for synthesis. And finally, a particular product may be in the focus of the studies and the goal is then to find the best way to prepare it.

Due to this broad portfolio of interests and needs, enzymes, substrates, products and methods that have been in the focus of the team over the years, it is essential to collaborate vividly and connect the knowledge of diverse fields. 

Current Projects

  • May 2017 - April 2020 FFG Industrienahe Dissertation:  Carboxylic Acid Reductases for Enzymatic Reduction of organic Acids to Aldehydes (CARERAA)

    In this project, we investigate CARs in depth with a focus on process relevant parameters.


  • Oct 2019 - Sept 2023 - CATalytic mechanisms and AppLications of OXidoreductases
    FWF Proj. Nr. 860262, Doc-Fund
    Partners: Prof. Robert Kourist, Prof. Rolf Breinbauer, Prof. Peter Macheroux, Prof. Bernd Nidetzky, Prof. Karl Gruber, Prof. Wolfgang Kroutil, AO Prof. Anton Glieder, Assoc.-Prof. Heidrun Gruber-Wölfler, Dr. Silvia Glück-Hartner
     
  • Jan 2020 - Dec 2023 - Enzymes for API Synthesis
    FFG COMET Project
    Partner: PharmaZell
    Collaborators: Prof. Helmut Schwab; Ass. Prof. Florian Rudroff, TU Wien; Dr. Gerhard G. Thallinger, Graz University of Technology
     
  • Jan 2020 - Dec 2023 - Engineering oxygenases for integrated bioprocesses
    FFG COMET Project
    Partner: bisy e.U.
    Collaborators: Prof. Gregorio Alvarez, University Autonoma Barcelona
     
  • Feb 2020 - Jan 2023 - Development of pyrazine producing strains
    Company Project
    Partners: Axxence Slovakia, s.r.o.
    Collaborators: Assistant Prof. Florian Rudroff, TUWien
     
  • Mar 2020 - Feb 2023 - A new chemoenzymatic route from carboxylic acids to nitriles
    FWF Proj. Nr. I 4607-N
    International Project
    Collaborators: Res. Prof. Ludmila Martinková, Dr. Miroslav Patek, Czech Academy of Sciences, Assistant Prof. Florian Rudroff, TUWien; Prof. Harald Gröger, University Bielefeld

Past Projects

  • November 2015 - February 2019 - FWF Project P 28477-B21: Catalytic cascades for selective aldehyde syntheses in vivo

    The main goal of this project is to understand the requirements and limitations of microbial cells for aldehyde synthesis. The synthesis of target products– chiral fragrance aldehydes – will be tackled by a combination of chemo-catalysis with a multi-enzymatic in vivo cascade reaction. This is a joint project with Dr. Florian Rudroff, Institute of Applied Synthetic Chemistry, TUVienna

    Project website:

    http://www.invivocascades.com
  • January 2017 - December 2018 - OeAD Project ZA 17/2017: Plant hydroxynitrile lyase diversity and application in industrial biocatalysis

    The goal of this project is to discover new enzymes from South African resources in collaboration with Prof. Karl Rumbold, University of the Witwatersrand, Johannesburg, South Africa.


  • April 2015 - March 2019 - Horizon2020 Project ROBOX: Grant agreement #635734


    The project title is Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols and our interest is to apply the combined knowledge and the analytical toolbox for the analysis of active pharmaceutical ingredients (APIs) to characterize oxidizing enzymes from the project partners.

    This project is carried out within acib in close collaboration with Prof. Anton Glieder (TUGraz) and DSM Chemical Technology R&D BV.

     

    The research for this work has received funding from the European Union (EU) project ROBOX (grant agreement n° 635734) under  EU’s Horizon 2020 Programme  Research and Innovation actions H2020-LEIT BIO-2014-1


  • January 2108 - December 2018 - OeAD ASEA/UNINET: Crystallization of fungal carboxylate reductase

    The goal of this project is to collaborate with Prof. Farah Diba Abu Bakar, University Kebangsaan Malaysia, Bangi, Malaysia.



  • March 2015 - February 2019 - FWF Elise-Richter-Fellowship V415-B21: Biocatalytic Carboxylate reduction

     

    The aim of this project is to get a better understanding of carboxylate reducing enzymes to enable the development of cell factories for the selective enzymatic preparation of aldehydes. We would like to know more about the structure-function relationship of this enzyme class and to identify more of these enzymes on amino acid level.


  • December 2012 - November 2015 - FP7 Project KYROBIO: Grant agreement no: 289646



    The project title is The discovery, development and demonstration of biocatalysts for use in the industrial synthesis of chiral chemicals and our interest was to identify a new class of hydroxynitrile lyases. We found a very promising enzyme in the fern Davallia teyermanii and characterized it in detail.

     

      This project was carried out within acib in close collaboration with Prof. Karl Gruber (UniGraz), Prof. Ruth Birner-Grünberger (ZMF and Omics Centre) and Dr. Gerhard Thallinger (TUGraz).

  • April 2014 - June 2015 - acib Project 84.011: Reverse nitrilase: From ammonium plus carboxylic acid to amine precursors

    The aim was to explore the substrate scope of enzymes that can convert a carboxylic acid to a nitrile.


  • January 2010 - December 2014 - acib Project 1.4-2: Enzymatic API Modification; Non-CYP drug metabolizing enzymes

    The aim was to make human drug metabolizing enzymes available in microbial hosts so that it is possible to prepare authentic human drug metabolites on the preparative scale.

    This project is carried out within acib in close collaboration with Novartis and F.Hoffmann-LaRoche.


  • January 2010 - December 2014 - acib Project 1.5-1: Amines and Peptides; Generation of efficient production strains for chiral amine synthesis

    The aim was to provide robust enzymes and yeast strains for the production of chiral amines.

    This project is carried out within acib in close collaboration with Prof. Anton Glieder, Dr. Andrea Camattari (TUGraz) and Ingenza Ltd.


  • January 2010 - December 2014 - acib Projects 1.2 and 4.12: Enzymatic Nitrile Reduction

    In this project we cloned, mutagenized and provided nitrile reductase enzymes to project partners with the aim to broaden its substrate specificity.

    This project is carried out within acib in close collaboration with Prof. Norbert Klempier, Prof. Bernd Nidetzky (TUGraz) and Prof. Karl Gruber (UniGraz).


  • January 2010 - June 2013 - acib Project 4.2-3: Enzyme engineering for complex reactions; Identification, expression and engineering of new complex microbial oxidoreductases

    The aim was to find enzymes for the reduction or oxidation of industrially relevant compounds.

    This project is carried out within acib in close collaboration with Prof. Helmut Schwab, Prof. Peter Macheroux (TUGraz) and Lonza AG.


  • January 2019 - July 2019 - FFG Proj. Nr. 870897 - FEM-Tech

    Collaborator: Dr. Gernot Strohmeier, Graz University of Technology

    The goal of this project is to synthesise aldehydes in cell free systems using carboxylate reductase enzymes. The need for stoichiometric amounts of ATP and NADPH is satisfied by recycling these costly cofactors using a cocktail of enzymes and polyphosphate as well as glucose as co-substrates.

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Current Theses

  • Melissa Horvat
    PhD thesis

    Working title: Carboxylic Acid Reductases for Enzymatic Reduction of organic Acids to Aldehydes

  • Daniel Schwendenwein
    PhD thesis

    Working title:Catalytic cascades for aldehyde synthesis in vivo
     
  • David Gradischnig
    Bachelor thesis
    Working title: Towards structure elucidation of Neurospora crassa CAR domains
     
  • Fritsche, Susanne:
    Working title: Characterization of CAR variants and new CARs
  • Steinberger, Stefan:
    Working title: Towards green leaf aldehydes from fatty acids by lipoxygenases and hydroperoxide lyases

  • Glänzer, Maximilian
    Working title: Characterization of novel nitrile hydratases

  • Schwarz, Anna
    Working title: In Vitro reduction of carboxylates to aldehydes

  • Hashem, Chiam
    PhD Thesis
    Systematic investigation of post-translational modification of carboxylic acid reductases

  • Steinberger, Stefan
    Master Thesis
    Towards green leaf aldehydes from fatty acids by lipoxygenases and hydroperoxide lyases

  • Tanja Saskia Larch
    Master thesis
    Systematic investigation of co-expression of carboxylate reductase and phosphopantetheinyl transferase in Pichia pastoris

  • Lukas Pfeifenberger
    Master thesis
    Identification and functional characterization of new peroxygenases

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Completed Theses

  • Philippe Oberhemm
    Master thesis

    Characterization of new carboxylate reductase enzymes from Neurospora crassa
    June 2018
     
  • Holly Stolterfoht
    Master thesis

    Mutational studies of Neurospora crassa carboxylate reductase
    November 2018
     
  • Bianca Kerschbaumer
    Master thesis

    Evaluation of CYP505 Variants for the Preparation of Drug Metabolites: Bridging from Analytical to Preparative Scale
    September 2018
     
  • Melissa Willim
    Master thesis

    CYP450 and its importance in Drug Development
    September 2018
     
  • Lena Gsöls
    Bachelor thesis

    Lyophilization of E. coli and Pichia pastoris strains expressing Cytochrome P450 enzymes
    2018

     
  • Bettina Schweda
    Bachelor Thesis

    Studies towards the Synthesis of Capsaicin and its Bioconversion using CYP 505
    January 2018
     
  • Lukas Kargl
    Bachelor thesis

    Kinetic characterization of CYP505 expressing biocatalysts
    October 2017

  • Katharina Dokulil
    Master Thesis

    Cloning, expression and characterization of 7-cyano-7-deazaguanine synthases
    May 2016
  • Thorsten Bachler
    Bachelor Thesis

    Human enzymes for organic synthesis
    April 2016

  • Elisa Lanfranchi
    PhD Thesis

    New Hydroxynitrile Lyase from white Rabbit's Foot Fern: The route from discovery to perspectives for applications
    Nov 2015
  • Kamila Napora-Wijata
    PhD Thesis

    Identification and expression of new microbial oxidoreductases
    February 2014
  • Thomas Prossliner
    Bachelor Thesis

    Characterization of a short-chain dehydrogenase/reductase from unconventional yeast Yarrowia lipolytica
    May 2013

Partners

Selected Publications

2021
Helmut Schwab Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members Show publication in PURE
Margit Winkler Cell-free in vitro reduction of carboxylates to aldehydes: with crude enzyme preparations to a key pharmaceutical building block Show publication in PURE
Helmut Schwab, Margit Winkler and Birgit Grill The sustainable synthesis of levetiracetam by an enzymatic dynamic kinetic resolution and an ex-cell anodic oxidation Show publication in PURE
Margit Winkler Production of the Carboxylate Reductase from Nocardia otitidiscaviarum in a Soluble, Active Form for in vitro Applications Show publication in PURE
2020
Robert Kourist Ground-State Destabilization by Active-Site Hydrophobicity Controls the Selectivity of a Cofactor-Free Decarboxylase Show publication in PURE
Anton Glieder Evolved Peroxygenase-Aryl Alcohol Oxidase Fusions for Self-Sufficient Oxyfunctionalization Reactions Show publication in PURE
Robert Kourist and Hanna Christiane Grimm Engineering of NADPH Supply Boosts Photosynthesis-Driven Biotransformations Show publication in PURE
P450 Show publication in PURE
Harald Pichler Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol Show publication in PURE
Robert Kourist A chemo-enzymatic tandem reaction in a mixture of deep eutectic solvent and water in continuous flow Show publication in PURE
Robert Kourist Cofactor Generation Cascade for α-Ketoglutarate and Fe(II)-Dependent Dioxygenases Show publication in PURE
Robert Kourist Solvent-Free Photobiocatalytic Hydroxylation of Cyclohexane Show publication in PURE
Anton Glieder Preparative-Scale Production of Testosterone Metabolites by Human Liver Cytochrome P450 Enzyme 3A4 Show publication in PURE
Margit Winkler, Petra Heidinger, Birgit Wiltschi, Martina Geier and Tamara Wriessnegger Enzymes revolutionize the bioproduction of value-added compounds Show publication in PURE
Harald Pichler and Robert Kourist Photobiocatalytic synthesis of chiral secondary fatty alcohols from renewable unsaturated fatty acids Show publication in PURE
Robert Kourist Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis Show publication in PURE
Anton Glieder Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris Show publication in PURE
Robert Kourist A combined experimental and modelling approach for the Weimberg pathway optimisation Show publication in PURE
Robert Kourist Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L Show publication in PURE
Karl Gruber Binding of dipeptidyl peptidase III to the oxidative stress cell sensor Kelch-like ECH-associated protein 1 is a two-step process Show publication in PURE
Helmut Schwab, Margit Winkler and Birgit Grill Functional Expression and Characterization of a Panel of Cobalt and Iron-Dependent Nitrile Hydratases Show publication in PURE
Harald Pichler Engineering of Saccharomyces cerevisiae for the production of (+)-ambrein Show publication in PURE
Margit Winkler and David Gradischnig Engineering carboxylic acid reductase for selective synthesis of medium-chain fatty alcohols in yeast Show publication in PURE
Margit Winkler and Melissa Horvat In vivo reduction of medium- to long-chain fatty acids by carboxylic acid reductase (CAR) enzymes: limitations and solutions Show publication in PURE
Margit Winkler and Daniel Schwendenwein High-throughput in-field bioprospecting for cyanogenic plants and hydroxynitrile lyases Show publication in PURE
Margit Winkler and Melissa Horvat Amino benzamidoxime (ABAO) based assay to identify efficient aldehyde-producing Pichia pastoris clones Show publication in PURE
Margit Winkler and Chiam Hashem Pyrazines: Synthesis and Industrial Application of these Valuable Flavor and Fragrance Compounds Show publication in PURE
Harald Pichler, Margit Winkler, Holly Stolterfoht, Chiam Hashem and Claudia Rinnofner Secretion of Pseudomonas Aeruginosa Lipoxygenase by Pichia Pastoris upon Glycerol Feed Show publication in PURE
Birgit Wiltschi Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose. Show publication in PURE
Birgit Wiltschi Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in E. coli. Show publication in PURE
Margit Winkler Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems Show publication in PURE
2019
Robert Kourist Using Deep Eutectic Solvents to Overcome Limited Substrate Solubility in the Enzymatic Decarboxylation of Bio-Based Phenolic Acids Show publication in PURE
Robert Kourist Artificial light-harvesting complexes enable Rieske oxygenase-catalyzed hydroxylations in non-photosynthetic cells Show publication in PURE
Rieske Non-Heme Iron Dioxygenases: Applications and Future PerspectivesEnzyme Catalysis: Perspectives on Basic & Applied Premises57-82 Show publication in PURE
Helmut Schwab, Harald Pichler and Anita Emmerstorfer-Augustin Pichia pastoris protease-deficient and auxotrophic strains generated by a novel, user-friendly vector toolbox for gene deletion Show publication in PURE
Anita Emmerstorfer-Augustin Analysis of the roles of phosphatidylinositol-4,5-bisphosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2 Show publication in PURE
Harald Pichler, Anita Emmerstorfer-Augustin and Matthias Engleder Exploring castellaniella defragrans linalool (De)hydratase-isomerase for enzymatic hydration of alkenes Show publication in PURE
Anton Glieder Single-cell approach to monitor the unfolded protein response during biotechnological processes with Pichia pastoris Show publication in PURE
Helmut Schwab A co-expression system to shift the equilibrium of transamination reactions toward the synthesis of enantiomerically pure amines Show publication in PURE
Robert Kourist Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase Show publication in PURE
Robert Kourist Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants Show publication in PURE
Karl Gruber and Birgit Wiltschi Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system Show publication in PURE
Helmut Schwab Enantioselective One-Pot Synthesis of Biaryl-Substituted Amines by Combining Palladium and Enzyme Catalysis in Deep Eutectic Solvents Show publication in PURE
Robert Kourist Preparation of optically pure flurbiprofen via an integrated chemo-enzymatic synthesis pathway Show publication in PURE
Robert Kourist One-Pot Transformation of Ketoximes into Optically Active Alcohols and Amines by Sequential Action of Laccases and Ketoreductases or ω-Transaminases Show publication in PURE
Helmut Schwab and Robert Kourist Amine Transaminase from Exophiala Xenobiotica - Crystal Structure and Engineering of a Fold IV Transaminase that Naturally Converts Biaryl Ketones Show publication in PURE
Harald Pichler and Tamara Wriessnegger Membrane protein production in yeast: Modification of yeast membranes for human membrane protein productionRecombinant Protein Production in Yeast: Methods and Protocols Show publication in PURE
Robert Kourist and Hanna Christiane Grimm Stereoselective Biotransformations of Cyclic Imines in Recombinant Cells of Synechocystis sp. PCC 6803 Show publication in PURE
Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization Show publication in PURE
Margit Winkler, Anton Glieder and Claudia Rinnofner Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst Show publication in PURE
Margit Winkler and Claudia Rinnofner Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila Show publication in PURE
Anton Glieder and Anna-Maria Hatzl Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies Show publication in PURE
Margit Winkler and Inge Eiteljörg Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH Show publication in PURE
Margit Winkler and Daniel Schwendenwein Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes Show publication in PURE
Margit Winkler, Robert Kourist and Melissa Horvat Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol Show publication in PURE
Helmut Schwab, Petra Heidinger and Robert Kourist Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator Show publication in PURE
Margit Winkler and Daniel Schwendenwein Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay Show publication in PURE
Margit Winkler and Melissa Horvat Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis Show publication in PURE
Margit Winkler A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus Show publication in PURE
2018
Harald Pichler Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine Show publication in PURE
Harald Pichler, Tamara Wriessnegger, Matthias Engleder and Melissa Horvat Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca Show publication in PURE
Harald Pichler Perdeuteration of cholesterol for neutron scattering applicatioins using recombinant Pichia pastoris Show publication in PURE
Margit Winkler, Holly Stolterfoht and Daniel Schwendenwein Identification of key residues for enzymatic carboxylate reduction Show publication in PURE
Harald Pichler Modification of Membrane Lipid Compositions in single-celled organisms–from Basics to Applications Show publication in PURE
Harald Pichler The Impact of Deuteration on Natural and Synthetic Lipids: a Neutron Diffraction Study Show publication in PURE
Library growth and protein expressionMethods in Molecular Biology145-156 Show publication in PURE
Biocatalytic oxidation reactions - a Chemist's perspective Show publication in PURE
Robert Kourist and Hanna Christiane Grimm Enzymatic Decarboxylation as a Tool for the Enzymatic Defunctionalization of Hydrophobic Bio-based Organic AcidsLipid Modification by Enzymes and Engineered Microbes89-118 Show publication in PURE
Harald Pichler and Matthias Engleder On the current role of hydratases in biocatalysis Show publication in PURE
MICROBIAL INDUCED ACID CORROSION FROM A FIELD PERSPECTIVE – ADVANCES IN PROCESS UNDERSTANDING AND CONSTRUCTION MATERIAL DEVELOPMENTProceedings of final conference of RILEM TC 253-MCI3-10 Show publication in PURE
Harald Pichler Whole-cell (+)-ambrein production in the yeast Pichia pastoris Show publication in PURE
Margit Winkler and Anton Glieder Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X Show publication in PURE
Margit Winkler Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090 Show publication in PURE
Anton Glieder and Anna-Maria Hatzl The Extreme Structural Plasticity in the CYP153 Subfamily of P450s Directs Development of Designer Hydroxylases Show publication in PURE
Phylogenomics uncovers early hybridization and adaptive loci shaping the radiation of Lake Tanganyika cichlid fishes Show publication in PURE
Robert Kourist Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis Show publication in PURE
Robert Kourist Raman Microspectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells Show publication in PURE
Anita Emmerstorfer-Augustin Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2 Show publication in PURE
Anton Glieder Parallelized biocatalytic scanning probe lithography for the additive fabrication of conjugated polymer structures Show publication in PURE
Robert Kourist Practical considerations regarding the choice of the best high-throughput assayMethods in Molecular Biology189-208 Show publication in PURE
Margit Winkler, Anton Glieder and Birgit Grill Production of Hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and Its Immobilization as a CLEA to Generate a Robust Biocatalyst Show publication in PURE
Robert Kourist Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE Show publication in PURE
Anton Glieder and Martina Geier Engineered bidirectional promoters enable rapid multi-gene co-expression optimization Show publication in PURE
Anton Glieder Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase Show publication in PURE
2017
Using Machine Learning and RFID Localization for Advanced Logistic Applications2017 IEEE Int. Conf. RFID73-74 Show publication in PURE
NR4A3 Suppresses Lymphomagenesis through Induction of Proapoptotic Genes Show publication in PURE
Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis Show publication in PURE
Robert Kourist Transcriptome Profiling of the Australian arid-land Plant Eremophila serrulata for the Identification of Monoterpene synthases Show publication in PURE
Robert Kourist Evolving Enzymes for BiocatalysisConsequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals1-17 Show publication in PURE
Margit Winkler, Holly Stolterfoht and Daniel Schwendenwein Four distinct types of E.C. 1.2.1.30 enzymes can catalyze the reduction of carboxylic acids to aldehydes Show publication in PURE
Helmut Schwab Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis Show publication in PURE
Karl Gruber, Margit Winkler and Anton Glieder Enzyme discovery beyond homology Show publication in PURE
Margit Winkler IN VIVO SYNTHESIS OF POLYHYDROXYLATED COMPOUNDS FROM A ‘HIDDEN RESERVOIR’ OF TOXIC ALDEHYDE SPECIES Show publication in PURE
Robert Kourist Overcoming the incompatibility challenge in chemo-enzymatic and multi-catalytic cascade reactions. Show publication in PURE
Robert Kourist Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803 Show publication in PURE
An alternative approach towards poly-epsilon-caprolactone through a chemoenzymatic synthesis: combined hydrogenation, bio-oxidations and polymerization without the isolation of intermediates Show publication in PURE
Photobiocatalytic alcohol oxidation using LED light sources Show publication in PURE
Towards environmentally acceptable synthesis of chiral alpha-hydroxy ketones via oxidase-lyase cascades Show publication in PURE
Process Development through Solvent Engineering in the Biocatalytic Synthesis of the Heterocyclic Bulk Chemical ε-Caprolactone Show publication in PURE
Robert Kourist Durch Trennen verbinden Show publication in PURE
Reconstruction of mitochondrial genotypes from diverse next generation sequencing datasetsProceedings of the 10th International joint conference on Biomedical Engineering Systems and Technologiesin press Show publication in PURE
Robert Kourist Practical Considerions Regarding the Choice of High-Throughput-AssaysProtein Engineering- Methods and Protocols189-208 Show publication in PURE
Robert Kourist BiocatalysisApplied Homogeneous Catalysis with Organometallic Compounds Show publication in PURE
Anton Glieder Pichia pastoris Alcohol Oxidase 1 (AOX1) Core Promoter Engineering by High Resolution Systematic Mutagenesis Show publication in PURE
Anton Glieder Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers Show publication in PURE
Anton Glieder Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris Show publication in PURE
Anton Glieder Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species Show publication in PURE
Karl Gruber, Margit Winkler and Anton Glieder Erratum Show publication in PURE
2016
Helmut Schwab Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold Show publication in PURE
Karl Gruber Structure of a Berberine Bridge Enzyme-Like Enzyme with an Active Site Specific to the Plant Family Brassicaceae Show publication in PURE
Whole genome sequence-based serogrouping of Listeria monocytogenes isolates Show publication in PURE
Oil sands tailings ponds harbour a small core prokaryotic microbiome and diverse accessory communities Show publication in PURE
Robert Kourist Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase Show publication in PURE
Karl Gruber An Esterase from Anaerobic Clostridium hathewayi Can Hydrolyze Aliphatic−Aromatic Polyesters Show publication in PURE
Special Issue on acib, Dedicated to the Occasion of Prof. Dr. Helmut Schwab's 65th Birthday Show publication in PURE
Robert Kourist Light-driven whole cell biotransformation with recombinant cyanobacteria Show publication in PURE
Carnivorous Nutrition in Pitcher Plants (Nepenthes spp.) via an Unusual Complement of Endogenous Enzymes Show publication in PURE
Margit Winkler TRAMETES VERSICOLOR CARBOXYLATE REDUCTASE UNCOVERED Show publication in PURE
Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease Show publication in PURE
Margit Winkler and Anton Glieder Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT) Show publication in PURE
Margit Winkler and Daniel Schwendenwein Selective Enzymatic Transformation to Aldehydes in vivo by Fungal Carboxylate Reductase from Neurospora crassa Show publication in PURE
Draft Genome Sequence of a 94-Year-Old Listeria monocytogenes Isolate, SLCC208 Show publication in PURE
Helmut Schwab and Petra Heidinger Characterization of two novel alcohol short-chain dehydrogenases/reductases from Ralstonia eutropha H16 capable of stereoselective conversion of bulky substrates Show publication in PURE
Harald Pichler and Tamara Wriessnegger Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions Show publication in PURE
Harald Pichler Oleate hydratase - structure function relationships and applicationPower of microbes in industry and environment33 Show publication in PURE
Molecular profiling of phagocytic immune cells in Anopheles gambiae reveals integral roles for hemocytes in mosquito innate immunity Show publication in PURE
Inferring expressed genes by whole-genome sequencing of plasma DNA Show publication in PURE
Curation of the genome annotation of Pichia pastoris (Komagataella phaffii) CBS7435 from gene level to protein function Show publication in PURE
Margit Winkler Human xanthine oxidase recombinant in E. coli: A whole cell catalyst for preparative drug metabolite synthesis Show publication in PURE
Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients Show publication in PURE
Translin and Trax differentially regulate telomere-associated transcript homeostasis Show publication in PURE
Harald Pichler and Anita Emmerstorfer-Augustin Screening for improved isoprenoid biosynthesis in microorganisms Show publication in PURE
Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time Show publication in PURE
Dimension reduction techniques for the integrative analysis of multi-omics data Show publication in PURE
Anton Glieder, Michaela Agnes Gerstmann, Martina Geier and Anna-Maria Hatzl A Toolbox of Diverse Promoters Related to Methanol Utilization Show publication in PURE
Helmut Schwab Methyltransferases Show publication in PURE
A Fed-Batch Synthetic Strategy for a Three-Step Enzymatic Synthesis of Poly-ϵ-caprolactone Show publication in PURE
Margit Winkler and Martina Geier Biocatalytic Characterization of Human FMO5: Unearthing Baeyer-Villiger Reactions in Humans Show publication in PURE
Anton Glieder and Michaela Agnes Gerstmann Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris Show publication in PURE
Anton Glieder and Anna-Maria Hatzl Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris Show publication in PURE
Anton Glieder Synergism of proteomics and mRNA sequencing for enzyme discovery Show publication in PURE
Birgit Wiltschi and Michaela Agnes Gerstmann Systems biocatalysis: para-alkenylation of unprotected phenols Show publication in PURE
Anton Glieder Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris Show publication in PURE
Harald Pichler and Anita Emmerstorfer-Augustin Production of aromatic plant terpenoids in recombinant baker´s yeastBiotechnology of Plant Secondary Metabolism79-89 Show publication in PURE
Helmut Schwab and Daniel Schwendenwein Enzymes as Biodevelopers for Nano- And Micropatterned Bicomponent Biopolymer Thin Films. Show publication in PURE
2015
Transcriptome Profiling of Khat (Catha edulis) and Ephedra sinica Reveals Gene Candidates Potentially Involved in Amphetamine-Type Alkaloid Biosynthesis. Show publication in PURE
Anton Glieder Novel DNA and RNA elementsSynthetic Biology65-99 Show publication in PURE
Anton Glieder and Michaela Agnes Gerstmann Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris. Show publication in PURE
Harald Pichler, Tamara Wriessnegger, Anita Emmerstorfer-Augustin and Matthias Engleder Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica Show publication in PURE
Robert Kourist Expression of enantioselective enzymes in cyanobacteria Show publication in PURE
Robert Kourist A New Class of Enzymes Discovered: A Non-Heme Oxidase Produces Medium-Chain 1-Alkenes Show publication in PURE
Robert Kourist A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts Show publication in PURE
Rhizobiales as functional and endosymbiontic members in the lichen symbiosis of Lobaria pulmonaria L. Show publication in PURE
Robert Kourist Photobiocatalytic decarboxylation for olefin synthesis Show publication in PURE
Robert Kourist Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance Show publication in PURE
α/β Hydrolase Domain-Containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate Show publication in PURE
Harald Pichler Sphingolipids regulate telomere clustering by affecting transcriptional levels of genes involved in telomere homeostasis Show publication in PURE
Oxidation of monolignols by members of the berberine bridge enzyme family suggests a role in plant cell wall metabolism Show publication in PURE
ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6 Show publication in PURE
Helmut Schwab and Anton Glieder Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris Show publication in PURE
Margit Winkler, Martina Geier and Thorsten Bachler Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis Show publication in PURE
Anton Glieder An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Show publication in PURE
MassBankEncyclopedia of Lipidomics1-3 Show publication in PURE
LincRNA-p21 acts as a mediator of ING1b-induced apoptosis Show publication in PURE
Margit Winkler The Nitrile forming Enzyme 7-Cyano-7-deazaguanine Synthase from Geobacillus kaustophilus: A reverse Nitrilase? Show publication in PURE
Anton Glieder Cyanohydrin Formation / Henry ReactionSience of Synthesis1-5 Show publication in PURE
Margit Winkler Drug Metabolite Synthesis with Human Enzymes Show publication in PURE
Anton Glieder and Martina Geier Compact multi-enzyme pathways in P. pastoris Show publication in PURE
Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants. Show publication in PURE
Eine Enzymkaskade zur Synthese von ε-Caprolacton und dessen Oligomeren Show publication in PURE
Karl Gruber and Helmut Schwab Improving the Properties of Bacterial R-selective Hydroxynitrile Lyases for Industrial Applications Show publication in PURE
LipidHomeEncyclopedia of Lipidomics1-3 Show publication in PURE
Helmut Schwab Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign Show publication in PURE
Margit Winkler and Anton Glieder Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection Show publication in PURE
Anton Glieder and Martina Geier Engineering Pichia pastoris for improved NADH regeneration Show publication in PURE
Anton Glieder A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris Show publication in PURE
Anton Glieder Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris Show publication in PURE
Helmut Schwab and Petra Heidinger Versatile plasmid-based expression systems for Gram-negative bacteria-General essentials exemplified with the bacterium Ralstonia eutropha H16. Show publication in PURE
Roles of Thermophiles and Fungi in Bitumen Degradation in Mostly Cold Oil Sands Outcrops Show publication in PURE
Comparative analysis of metagenomes from three methanogenic hydrocarbon-degrading enrichment cultures with 41 environmental samples. Show publication in PURE
Margit Winkler and Birgit Wiltschi Fluoro amino acids: A rarity in nature, yet a prospect for protein engineering Show publication in PURE
Helmut Schwab Oxidative Alkene Cleavage Catalysed by Manganese-​Dependent Cupin TM1459 from Thermotoga maritima Show publication in PURE
LIPID MAPS: Tools and DatabasesEncyclopedia of Lipidomics Show publication in PURE
Helmut Schwab, Harald Pichler, Tamara Wriessnegger and Anita Emmerstorfer-Augustin Over-expression of ICE2 stabilizes cytochrome P450 reductase levels in Saccharomyces cerevisiae and Pichia pastoris Show publication in PURE
2014
Helmut Schwab High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities Show publication in PURE
Anton Glieder Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD (P)+ Regeneration in Biocatalytic Oxidations Show publication in PURE
Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation Show publication in PURE
Anton Glieder Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii Show publication in PURE
Helmut Schwab Mechanistic study of CMP-Neu5Ac hydrolysis by α2,3-sialyltransferase from Pasteurella dagmatis Show publication in PURE
Helmut Schwab and Petra Heidinger Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16 Show publication in PURE
Anton Glieder Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression Show publication in PURE
Robert Kourist Production of macrocyclic sesqui‐and diterpenes in heterologous microbial hosts: A systems approach to harness Nature’s molecular diversity Show publication in PURE
Robert Kourist Enzymatic decarboxylation—an emerging reaction for chemicals production from renewable resources Show publication in PURE
Margit Winkler and Anton Glieder Carbon source dependent promoters in yeasts Show publication in PURE
Robert Kourist Lipases as Sustainable Biocatalysts for the Sustainable Industrial Production of Fine Chemicals and Cosmetics Show publication in PURE
Margit Winkler, Anton Glieder and Thorsten Bachler PRODUCTION OF RECOMBINANT HUMAN ALDEHYDE OXIDASE IN E. COLI AND OPTIMIZATION OF ITS APPLICATION FOR PREPARATIVE SYNTHESIS OF OXIDIZED DRUG METABOLITES Show publication in PURE
Methyltransferases in biocatalysisCascade Biocatalysis: Stereoselective and Environmentally Friendly Reactions Show publication in PURE
Helmut Schwab Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations Show publication in PURE
MicroRNA-26 family is required for human adipogenesis and drives brown adipocytes characteristics Show publication in PURE
Robert Kourist Microbial Lactone Synthesis Based on Renewable Resources Show publication in PURE
Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases. Show publication in PURE
Anton Glieder Microbials for the production of monoclonal antibodies and antibody fragments Show publication in PURE
Improved selectivity of an engineered multi-product terpene synthase Show publication in PURE
Harald Pichler, Tamara Wriessnegger and Anita Emmerstorfer-Augustin Overexpression of membrane proteins from higher eukaryotes in yeasts Show publication in PURE
Anton Glieder Peroxidase gene discovery from the horseradish transcriptome Show publication in PURE
Margit Winkler and Anton Glieder Extracellular transaminases for biocatalysisNew BiotechnologyS198-S198 Show publication in PURE
Margit Winkler Unconventional Enzymatic Reductions: Nitriles and Carboxylates as SubstratesNew BiotechnologyS1-S2 Show publication in PURE
Margit Winkler, Anton Glieder, Martina Geier and Thorsten Bachler Human flavin monooxygenase 2: Heterologous expression in E. coli and API modificationNew BiotechnologyS82-S82 Show publication in PURE
MicroRNA functions in brite/brown fat - novel perspectives towards anti-obesity strategies Show publication in PURE
MicroRNA functions in human fat cell development - Novel perspectives towards anti-obesity strategiesBIT´s Annual World Congress of Molecular & Cell Biology - 2014124-124 Show publication in PURE
Anton Glieder Enantioselective trans-dihydroxylation of aryl olefins by cascade biocatalysis with recombinant escherichia coli coexpressing monooxygenase and epoxide hydrolase Show publication in PURE
Anton Glieder and Claudia Rinnofner Synthetic Core Promoters for Pichia pastoris Show publication in PURE
MiR-200a regulates epithelial to mesenchymal transition-related gene expression and determines prognosis in colorectal cancer patients Show publication in PURE
Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation Show publication in PURE
Anton Glieder Discovery of fungal enzymes and pathwaysFungal Bio-Molecules: Sources, Applications and Recent Developments51-61 Show publication in PURE
Karl Gruber Collapse of the native structure caused by a single amino acid exchange in human NAD(P)H:quinone oxidoreductase1 Show publication in PURE
Margit Winkler BIOCATALYTIC REDUCTION OF CARBOXYLIC ACIDS Show publication in PURE
Helmut Schwab Crystal structure of an (R)-selective ω-transaminase from Aspergillus terreus (R)-selective ω-transaminase from Aspergillus terre Show publication in PURE
Helmut Schwab Discovery of a novel (R)-selective bacterial cupin HNL from Acidobacterium capsulatum ATCC 51196 by a sequence-based approach Show publication in PURE
NR4A1-mediated apoptosis supresses lymphomagenesis and is associated with a favorable cancer specific survival in patients with aggressive B-cell lymphomas Show publication in PURE
Anton Glieder Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks Show publication in PURE
Anton Glieder Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris. Show publication in PURE
Helmut Schwab, Harald Pichler, Tamara Wriessnegger and Matthias Engleder Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastors Show publication in PURE
Hunting the needle in the haystack - A guide to obtain biologically meaningful microRNA targets Show publication in PURE
Margit Winkler SEMI-PREPARATIVE SCALE DRUG METABOLITE SYNTHESIS WITH HUMAN FLAVIN MONOOXYGENASESPractical Methods in Biocatalysis and Biotransformations135-139 Show publication in PURE
Helmut Schwab and Harald Pichler Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production Show publication in PURE
2013
Artificial Multi-Enzyme Networks for the Asymmetric Amination of sec-Alcohols Show publication in PURE
Robert Kourist Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants Show publication in PURE
Karl Gruber, Harald Pichler and Anita Emmerstorfer-Augustin Enzymatic aerobic alkene cleavage catalyzed by a Mn3+-dependent proteinase A homologue Show publication in PURE
Harald Pichler and Tamara Wriessnegger A novel cholesterol-producing Pichia pastoris strain is an ideal host for functional expression of human Na,K-ATPase α3β1 isoform Show publication in PURE
Helmut Schwab Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis Show publication in PURE
Development of small-molecule inhibitors targeting adipose triglyceride lipase Show publication in PURE
Helmut Schwab and Petra Heidinger Metagenome analyses reveal the influence of the inoculant Lactobacillus buchneri CD034 on the microbial community involved in grass ensiling Show publication in PURE
Margit Winkler YARROWIA LIPOLYTICA DEHYDROGENASE/ REDUCTASE: AN ENZYME TOLERANT FOR LIPOPHILIC COMPOUNDS AND CARBOHYDRATE SUBSTRATES Show publication in PURE
Karl Gruber and Helmut Schwab Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis Show publication in PURE
Martina Geier Protein Engineering Using Eukaryotic Expression SystemsProtein Engineering Handbook - Volume 347-72 Show publication in PURE
Helmut Schwab and Harald Pichler Probing the secretory pathway of the yeast Pichia pastorisBook of abstracts38-38 Show publication in PURE
Helmut Schwab, Harald Pichler, Tamara Wriessnegger and Anita Emmerstorfer-Augustin Terpenoid production in yeastsBook of abstracts26-26 Show publication in PURE
Karl Gruber and Margit Winkler Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering Show publication in PURE
Margit Winkler and Anton Glieder RECENT DEVELOPMENTS IN HYDROXYNITRILE LYASES FOR INDUSTRIAL BIOTECHNOLOGY Show publication in PURE
New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris Show publication in PURE
Helmut Schwab Investigation of one-enzyme systems in the w-transaminase-catalyzed synthesis of chiral amines Show publication in PURE
Anton Glieder and Martina Geier First functional expression of cytochrome P450 3A4 in Pichia pastoris Show publication in PURE
Regulation of Pichia pastoris promoters and its consequences for protein production Show publication in PURE
Karl Gruber and Helmut Schwab Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase Show publication in PURE
Karl Gruber and Helmut Schwab Fusion of binding-domains to Thermobifida cellulosilytica cutinase to tune sorption characteristics and enhancing PET hydrolysis Show publication in PURE
Anton Glieder and Martina Geier Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation Show publication in PURE
Anton Glieder Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris Show publication in PURE
2012
Margit Winkler Organ Function in Shake Flasks: Recombinant Human Enzymes for the Pharmaceutical Industry Show publication in PURE
Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates Show publication in PURE
Anton Glieder Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway Show publication in PURE
Robert Kourist The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity Show publication in PURE
Helmut Schwab and Inge Eiteljörg Characterization of a new cutinase from Thermobifida alba regarding PET-surface hydrolysis Show publication in PURE
Anton Glieder and Martina Geier MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes Show publication in PURE
High-throughput characterization and comparison of microbial communitiesComputational Medicine37-57 Show publication in PURE
Robert Kourist Creation of a lipase highly selective for trans fatty acids by protein engineering Show publication in PURE
Robert Kourist Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols Show publication in PURE
Margit Winkler Synthesis of Fluorinated Neurotransmitter AnaloguesFluorine in Pharmaceutical and Medicinal Chemistry : From Biophysical Aspects to Clinical Applications299-331 Show publication in PURE
Helmut Schwab and Inge Eiteljörg Extracellular serine proteases from Stenotrophomonas maltophilia: screening, isolation and heterologous expression in E.coli. Show publication in PURE
Margit Winkler and Anton Glieder C–X Bond Formation: Hydroxynitrile lyases: From Nature to ApplicationComprehensive Chirality350-371 Show publication in PURE
Karl Gruber and Helmut Schwab A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA) Show publication in PURE
Anton Glieder and Martina Geier Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes Show publication in PURE
Margit Winkler and Anton Glieder Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale Show publication in PURE
Margit Winkler and Anton Glieder NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION Show publication in PURE
Helmut Schwab Crystallisation of the novel S-adenosyl-L-methionine dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. Show publication in PURE
Harald Pichler and Tamara Wriessnegger Pichia pastoris as whole-cell biocatalyst for (+)-nootkatone productionPichia 201248-48 Show publication in PURE
Margit Winkler and Anton Glieder A NOVEL MULTI-ENZYMATIC ASSAY FOR TRANSAMINASE ACTIVITY: HIGH THROUGHPUT SCREENING APPLICATIONS IN PICHIA PASTORIS Show publication in PURE
Harald Pichler, Anton Glieder, Martina Geier and Anita Emmerstorfer-Augustin Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study Show publication in PURE
Margit Winkler Recombinant Human Flavin Monooxygenases: Expression and Moclobemide N-oxide Synthesis on Semi-Preparative ScaleNew BiotechnologyS236-S237 Show publication in PURE
Helmut Schwab Crystallization of a novel metal-containing cupin from Acidobacterium sp. and preliminary diffraction data analysis Show publication in PURE
Anton Glieder Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris Show publication in PURE
Organaktivitäten im Schüttelkolben: Rekombinante menschliche Enzyme für die Pharmaindustrie Show publication in PURE
Helmut Schwab Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage Show publication in PURE
Anton Glieder and Claudia Rinnofner Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology Show publication in PURE
Anton Glieder Expression of lignocellulolytic enzymes in Pichia pastoris Show publication in PURE
Anton Glieder Sensitive high-throughput screening for the detection of reducing sugars Show publication in PURE
Helmut Schwab Two-step enzymatic functionalisation of polyamide with phenolics Show publication in PURE
Helmut Schwab Recent advances in rational approaches for enzyme engineerings in rational approaches for enzyme engineering Show publication in PURE
Helmut Schwab Characterization of two bacterial hydroxynitrile lyases with high similarity to cupin superfamily proteins Show publication in PURE
Helmut Schwab and Inge Eiteljörg Molecular characterization of the C-Methyltransferase NovO of Streptomyces spheroides, a valuable enzyme for performing Friedel-Crafts alkylation Show publication in PURE
2011
Margit Winkler and Anton Glieder HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS Show publication in PURE
Robert Kourist One-step enzyme extraction and immobilization for biocatalysis applications. Show publication in PURE
Robert Kourist Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries. Show publication in PURE
Robert Kourist Biocatalytic synthesis of optically active tertiary alcohols Show publication in PURE
Robert Kourist Biocatalytic strategies for the asymmetric synthesis of profens – recent trends and developments Show publication in PURE
Robert Kourist Protein engineering of arylmalonate decarboxylase G74C, an artificial racemase Show publication in PURE
Robert Kourist Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution. Show publication in PURE
Robert Kourist Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining Show publication in PURE
Helmut Schwab and Inge Eiteljörg Enzymatic Surface Hydrolysis of PET. Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida Show publication in PURE
Petra Heidinger The cadherin switch in melanoma instigated by HGF is mediated through epithelial-mesenchymal transition regulators. Show publication in PURE
Harald Pichler A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance Show publication in PURE
Margit Winkler and Anton Glieder Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones Show publication in PURE
Anton Glieder Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3 Show publication in PURE
Petra Heidinger Transcriptional Activation of ZEB1 by Slug Leads to Cooperative Regulation of the EMT like Phenotype in Melanoma Show publication in PURE
Helmut Schwab and Inge Eiteljörg Hydrolysis of polyethyleneterephthalate by para-nitrobenzylesterase from Bacillus subtilis Show publication in PURE
Helmut Schwab, Harald Pichler and Anton Glieder High-quality genome sequence of Pichia pastoris CBS7435 Show publication in PURE
2010
Robert Kourist An Enzymatic Toolbox for the Kinetic Resolution of 2-(Pyridin- x -yl)but-3-yn-2-ols and Tertiary Cyanohydrins Show publication in PURE
Robert Kourist Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation Show publication in PURE
Anton Glieder Stepwise engineering of a Pichia pastoris D-amino acid whole cell catalyst Show publication in PURE
Petra Heidinger HGF-promoted motility in primary human melanocytes depends on CD44v6 regulated via NF-kappa B, Egr-1, and C/EBP-beta Show publication in PURE
Margit Winkler IMPROVED FITNESS OF ARABIDOPSIS THALIANA NITRILASE 2 Show publication in PURE
Karl Gruber, Helmut Schwab and Inge Eiteljörg C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli. Show publication in PURE
Anton Glieder and Claudia Rinnofner Perspectives on synthetic promoters for biocatalysis and biotransformation Show publication in PURE
Microbial PHA Production from Waste Raw MaterialsPlastics from Bacteria: Natural Functions and Applications85-119 Show publication in PURE
Margit Winkler and Anton Glieder Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications Show publication in PURE
Anton Glieder Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation Show publication in PURE
Robert Kourist Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases. Show publication in PURE
Margit Winkler and Anton Glieder Directed evolution of Alcaligenes faecalis nitrilase Show publication in PURE
Helmut Schwab and Inge Eiteljörg Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents. Show publication in PURE
Robert Kourist The role of the GGGX motif in determining the activity and enantioselectivity of pig liver esterase towards tertiary alcohols Show publication in PURE
Anton Glieder Real time PCR based determination of gene copy numbers in Pichia pastoris Show publication in PURE
Robert Kourist Protein engineering and discovery of lipases Show publication in PURE
Robert Kourist The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering Show publication in PURE

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Team Leader
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Margit Winkler
Dipl.-Ing. Dr.techn.