Winkler Team
Past Projects
- November 2015 - February 2019 - FWF Project P 28477-B21: Catalytic cascades for selective aldehyde syntheses in vivo
The main goal of this project is to understand the requirements and limitations of microbial cells for aldehyde synthesis. The synthesis of target products– chiral fragrance aldehydes – will be tackled by a combination of chemo-catalysis with a multi-enzymatic in vivo cascade reaction. This is a joint project with Dr. Florian Rudroff, Institute of Applied Synthetic Chemistry, TUVienna
Project website:
http://www.invivocascades.com - January 2017 - December 2018 - OeAD Project ZA 17/2017: Plant hydroxynitrile lyase diversity and application in industrial biocatalysis
The goal of this project is to discover new enzymes from South African resources in collaboration with Prof. Karl Rumbold, University of the Witwatersrand, Johannesburg, South Africa.
- April 2015 - March 2019 - Horizon2020 Project ROBOX: Grant agreement #635734
The project title is Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols and our interest is to apply the combined knowledge and the analytical toolbox for the analysis of active pharmaceutical ingredients (APIs) to characterize oxidizing enzymes from the project partners.This project is carried out within acib in close collaboration with Prof. Anton Glieder (TUGraz) and DSM Chemical Technology R&D BV.
The research for this work has received funding from the European Union (EU) project ROBOX (grant agreement n° 635734) under EU’s Horizon 2020 Programme Research and Innovation actions H2020-LEIT BIO-2014-1 
- January 2108 - December 2018 - OeAD ASEA/UNINET: Crystallization of fungal carboxylate reductase
The goal of this project is to collaborate with Prof. Farah Diba Abu Bakar, University Kebangsaan Malaysia, Bangi, Malaysia.
- March 2015 - February 2019 - FWF Elise-Richter-Fellowship V415-B21: Biocatalytic Carboxylate reduction
The aim of this project is to get a better understanding of carboxylate reducing enzymes to enable the development of cell factories for the selective enzymatic preparation of aldehydes. We would like to know more about the structure-function relationship of this enzyme class and to identify more of these enzymes on amino acid level.
- December 2012 - November 2015 - FP7 Project KYROBIO: Grant agreement no: 289646
The project title is The discovery, development and demonstration of biocatalysts for use in the industrial synthesis of chiral chemicals and our interest was to identify a new class of hydroxynitrile lyases. We found a very promising enzyme in the fern Davallia teyermanii and characterized it in detail.This project was carried out within acib in close collaboration with Prof. Karl Gruber (UniGraz), Prof. Ruth Birner-Grünberger (ZMF and Omics Centre) and Dr. Gerhard Thallinger (TUGraz). - April 2014 - June 2015 - acib Project 84.011: Reverse nitrilase: From ammonium plus carboxylic acid to amine precursors
The aim was to explore the substrate scope of enzymes that can convert a carboxylic acid to a nitrile.
- January 2010 - December 2014 - acib Project 1.4-2: Enzymatic API Modification; Non-CYP drug metabolizing enzymes
The aim was to make human drug metabolizing enzymes available in microbial hosts so that it is possible to prepare authentic human drug metabolites on the preparative scale.
This project is carried out within acib in close collaboration with Novartis and F.Hoffmann-LaRoche.
- January 2010 - December 2014 - acib Project 1.5-1: Amines and Peptides; Generation of efficient production strains for chiral amine synthesis
The aim was to provide robust enzymes and yeast strains for the production of chiral amines.
This project is carried out within acib in close collaboration with Prof. Anton Glieder, Dr. Andrea Camattari (TUGraz) and Ingenza Ltd.
- January 2010 - December 2014 - acib Projects 1.2 and 4.12: Enzymatic Nitrile Reduction
In this project we cloned, mutagenized and provided nitrile reductase enzymes to project partners with the aim to broaden its substrate specificity.
This project is carried out within acib in close collaboration with Prof. Norbert Klempier, Prof. Bernd Nidetzky (TUGraz) and Prof. Karl Gruber (UniGraz).
- January 2010 - June 2013 - acib Project 4.2-3: Enzyme engineering for complex reactions; Identification, expression and engineering of new complex microbial oxidoreductases
The aim was to find enzymes for the reduction or oxidation of industrially relevant compounds.
This project is carried out within acib in close collaboration with Prof. Helmut Schwab, Prof. Peter Macheroux (TUGraz) and Lonza AG.
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January 2019 - July 2019 - FFG Proj. Nr. 870897 - FEM-Tech
Collaborator: Dr. Gernot Strohmeier, Graz University of Technology
The goal of this project is to synthesise aldehydes in cell free systems using carboxylate reductase enzymes. The need for stoichiometric amounts of ATP and NADPH is satisfied by recycling these costly cofactors using a cocktail of enzymes and polyphosphate as well as glucose as co-substrates.
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Selected Publications
2020
Enzymes revolutionize the bioproduction of value-added compounds
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High-throughput in-field bioprospecting for cyanogenic plants and hydroxynitrile lyases
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Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems
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Engineering of Saccharomyces cerevisiae for the production of (+)-ambrein
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A chemo-enzymatic tandem reaction in a mixture of deep eutectic solvent and water in continuous flow
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2019
Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization
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Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst
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Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila
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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
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Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH
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Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes
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Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol
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Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay
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Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator
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Stereoselective Biotransformations of Cyclic Imines in Recombinant Cells of Synechocystis sp. PCC 6803
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Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis
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A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus
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Rieske Non-Heme Iron Dioxygenases: Applications and Future PerspectivesRieske Non-Heme Iron Dioxygenases: Applications and Future Perspectives57-82
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Pichia pastoris protease-deficient and auxotrophic strains generated by a novel, user-friendly vector toolbox for gene deletion
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Analysis of the roles of phosphatidylinositol-4,5-bisphosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2
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Exploring castellaniella defragrans linalool (De)hydratase-isomerase for enzymatic hydration of alkenes
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Single-cell approach to monitor the unfolded protein response during biotechnological processes with Pichia pastoris
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A co-expression system to shift the equilibrium of transamination reactions toward the synthesis of enantiomerically pure amines
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Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase
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Using Deep Eutectic Solvents to Overcome Limited Substrate Solubility in the Enzymatic Decarboxylation of Bio-Based Phenolic Acids
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Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants
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Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system
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Enantioselective One-Pot Synthesis of Biaryl-Substituted Amines by Combining Palladium and Enzyme Catalysis in Deep Eutectic Solvents
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Preparation of optically pure flurbiprofen via an integrated chemo-enzymatic synthesis pathway
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One-Pot Transformation of Ketoximes into Optically Active Alcohols and Amines by Sequential Action of Laccases and Ketoreductases or ω-Transaminases
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Amine Transaminase from Exophiala Xenobiotica - Crystal Structure and Engineering of a Fold IV Transaminase that Naturally Converts Biaryl Ketones
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Artificial light-harvesting complexes enable Rieske oxygenase-catalyzed hydroxylations in non-photosynthetic cells
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Membrane protein production in yeast: Modification of yeast membranes for human membrane protein productionMembrane protein production in yeast: Modification of yeast membranes for human membrane protein production
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2018
The Extreme Structural Plasticity in the CYP153 Subfamily of P450s Directs Development of Designer Hydroxylases
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Phylogenomics uncovers early hybridization and adaptive loci shaping the radiation of Lake Tanganyika cichlid fishes
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Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis
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Raman Microspectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells
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Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2
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Parallelized biocatalytic scanning probe lithography for the additive fabrication of conjugated polymer structures
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Practical considerations regarding the choice of the best high-throughput assayPractical considerations regarding the choice of the best high-throughput assay189-208
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Production of Hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and Its Immobilization as a CLEA to Generate a Robust Biocatalyst
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Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE
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Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase
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Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine
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Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca
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Perdeuteration of cholesterol for neutron scattering applicatioins using recombinant Pichia pastoris
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Identification of key residues for enzymatic carboxylate reduction
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Modification of Membrane Lipid Compositions in single-celled organisms–from Basics to Applications
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The Impact of Deuteration on Natural and Synthetic Lipids: a Neutron Diffraction Study
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Library growth and protein expressionLibrary growth and protein expression145-156
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Biocatalytic oxidation reactions - a Chemist's perspective
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Enzymatic Decarboxylation as a Tool for the Enzymatic Defunctionalization of Hydrophobic Bio-based Organic AcidsEnzymatic Decarboxylation as a Tool for the Enzymatic Defunctionalization of Hydrophobic Bio-based Organic Acids89-118
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On the current role of hydratases in biocatalysis
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MICROBIAL INDUCED ACID CORROSION FROM A FIELD PERSPECTIVE – ADVANCES IN PROCESS UNDERSTANDING AND CONSTRUCTION MATERIAL DEVELOPMENTMICROBIAL INDUCED ACID CORROSION FROM A FIELD PERSPECTIVE – ADVANCES IN PROCESS UNDERSTANDING AND CONSTRUCTION MATERIAL DEVELOPMENT3-10
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Whole-cell (+)-ambrein production in the yeast Pichia pastoris
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Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X
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Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090
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2017
Overcoming the incompatibility challenge in chemo-enzymatic and multi-catalytic cascade reactions.
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Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803
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Photobiocatalytic alcohol oxidation using LED light sources
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An alternative approach towards poly-epsilon-caprolactone through a chemoenzymatic synthesis: combined hydrogenation, bio-oxidations and polymerization without the isolation of intermediates
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Towards environmentally acceptable synthesis of chiral alpha-hydroxy ketones via oxidase-lyase cascades
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Process Development through Solvent Engineering in the Biocatalytic Synthesis of the Heterocyclic Bulk Chemical ε-Caprolactone
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Durch Trennen verbinden
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Reconstruction of mitochondrial genotypes from diverse next generation sequencing datasetsReconstruction of mitochondrial genotypes from diverse next generation sequencing datasetsin press
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Practical Considerions Regarding the Choice of High-Throughput-AssaysPractical Considerions Regarding the Choice of High-Throughput-Assays189-208
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Pichia pastoris Alcohol Oxidase 1 (AOX1) Core Promoter Engineering by High Resolution Systematic Mutagenesis
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Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers
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Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris
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Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species
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Erratum
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Using Machine Learning and RFID Localization for Advanced Logistic ApplicationsUsing Machine Learning and RFID Localization for Advanced Logistic Applications73-74
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NR4A3 Suppresses Lymphomagenesis through Induction of Proapoptotic Genes
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Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis
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Transcriptome Profiling of the Australian arid-land Plant Eremophila serrulata for the Identification of Monoterpene synthases
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Four distinct types of E.C. 1.2.1.30 enzymes can catalyze the reduction of carboxylic acids to aldehydes
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Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis
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Enzyme discovery beyond homology
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IN VIVO SYNTHESIS OF POLYHYDROXYLATED COMPOUNDS FROM A ‘HIDDEN RESERVOIR’ OF TOXIC ALDEHYDE SPECIES
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2016
Oleate hydratase - structure function relationships and applicationOleate hydratase - structure function relationships and application33
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Molecular profiling of phagocytic immune cells in Anopheles gambiae reveals integral roles for hemocytes in mosquito innate immunity
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Inferring expressed genes by whole-genome sequencing of plasma DNA
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Curation of the genome annotation of Pichia pastoris (Komagataella phaffii) CBS7435 from gene level to protein function
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Human xanthine oxidase recombinant in E. coli: A whole cell catalyst for preparative drug metabolite synthesis
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Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients
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Translin and Trax differentially regulate telomere-associated transcript homeostasis
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Screening for improved isoprenoid biosynthesis in microorganisms
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Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time
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Dimension reduction techniques for the integrative analysis of multi-omics data
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A Toolbox of Diverse Promoters Related to Methanol Utilization
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Methyltransferases
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A Fed-Batch Synthetic Strategy for a Three-Step Enzymatic Synthesis of Poly-ϵ-caprolactone
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Biocatalytic Characterization of Human FMO5: Unearthing Baeyer-Villiger Reactions in Humans
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Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris
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Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris
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Synergism of proteomics and mRNA sequencing for enzyme discovery
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Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris
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Production of aromatic plant terpenoids in recombinant baker´s yeastProduction of aromatic plant terpenoids in recombinant baker´s yeast79-89
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Enzymes as Biodevelopers for Nano- And Micropatterned Bicomponent Biopolymer Thin Films.
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Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold
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Structure of a Berberine Bridge Enzyme-Like Enzyme with an Active Site Specific to the Plant Family Brassicaceae
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Whole genome sequence-based serogrouping of Listeria monocytogenes isolates
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Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen
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Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase
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Oil sands tailings ponds harbour a small core prokaryotic microbiome and diverse accessory communities
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An Esterase from Anaerobic Clostridium hathewayi Can Hydrolyze Aliphatic−Aromatic Polyesters
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Lichtgetriebene Ganzzellbiotransformation mit rekombinanten Cyanobakterien
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Special Issue on acib, Dedicated to the Occasion of Prof. Dr. Helmut Schwab's 65th Birthday
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Carnivorous Nutrition in Pitcher Plants (Nepenthes spp.) via an Unusual Complement of Endogenous Enzymes
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TRAMETES VERSICOLOR CARBOXYLATE REDUCTASE UNCOVERED
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Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease
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Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT)
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Selective Enzymatic Transformation to Aldehydes in vivo by Fungal Carboxylate Reductase from Neurospora crassa
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Draft Genome Sequence of a 94-Year-Old Listeria monocytogenes Isolate, SLCC208
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Characterization of two novel alcohol short-chain dehydrogenases/reductases from Ralstonia eutropha H16 capable of stereoselective conversion of bulky substrates
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Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions
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2015
An updated view on horseradish peroxidases: recombinant production and biotechnological applications.
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LincRNA-p21 acts as a mediator of ING1b-induced apoptosis
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Drug Metabolite Synthesis with Human Enzymes
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The Nitrile forming Enzyme 7-Cyano-7-deazaguanine Synthase from Geobacillus kaustophilus: A reverse Nitrilase?
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Cyanohydrin Formation / Henry ReactionCyanohydrin Formation / Henry Reaction1-5
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Compact multi-enzyme pathways in P. pastoris
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Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants.
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Eine Enzymkaskade zur Synthese von ε-Caprolacton und dessen Oligomeren
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Improving the Properties of Bacterial R-selective Hydroxynitrile Lyases for Industrial Applications
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Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign
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Engineering Pichia pastoris for improved NADH regeneration
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Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection
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A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris
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Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris
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Roles of Thermophiles and Fungi in Bitumen Degradation in Mostly Cold Oil Sands Outcrops
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Versatile plasmid-based expression systems for Gram-negative bacteria-General essentials exemplified with the bacterium Ralstonia eutropha H16.
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Comparative analysis of metagenomes from three methanogenic hydrocarbon-degrading enrichment cultures with 41 environmental samples.
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FLUORINATED AMINO ACIDS – A RARITY IN NATURE YET A PROSPECT FOR PROTEIN ENGINEERING
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Over-expression of ICE2 stabilizes cytochrome P450 reductase levels in Saccharomyces cerevisiae and Pichia pastoris
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Oxidative Alkene Cleavage Catalysed by Manganese-Dependent Cupin TM1459 from Thermotoga maritima
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Transcriptome Profiling of Khat (Catha edulis) and Ephedra sinica Reveals Gene Candidates Potentially Involved in Amphetamine-Type Alkaloid Biosynthesis.
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Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris.
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Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica
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Expression of enantioselective enzymes in cyanobacteria
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A New Class of Enzymes Discovered: A Non-Heme Oxidase Produces Medium-Chain 1-Alkenes
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A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts
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Rhizobiales as functional and endosymbiontic members in the lichen symbiosis of Lobaria pulmonaria L.
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Photobiocatalytic decarboxylation for olefin synthesis
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Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance
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Sphingolipids regulate telomere clustering by affecting transcriptional levels of genes involved in telomere homeostasis
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α/β Hydrolase Domain-Containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate
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Oxidation of monolignols by members of the berberine bridge enzyme family suggests a role in plant cell wall metabolism
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ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6
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Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
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Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis
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2014
Peroxidase gene discovery from the horseradish transcriptome
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MicroRNA functions in human fat cell development - Novel perspectives towards anti-obesity strategiesMicroRNA functions in human fat cell development - Novel perspectives towards anti-obesity strategies124-124
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MicroRNA functions in brite/brown fat - novel perspectives towards anti-obesity strategies
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Enantioselective trans-dihydroxylation of aryl olefins by cascade biocatalysis with recombinant escherichia coli coexpressing monooxygenase and epoxide hydrolase
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Synthetic Core Promoters for Pichia pastoris
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MiR-200a regulates epithelial to mesenchymal transition-related gene expression and determines prognosis in colorectal cancer patients
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Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation
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Discovery of fungal enzymes and pathwaysDiscovery of fungal enzymes and pathways51-61
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BIOCATALYTIC REDUCTION OF CARBOXYLIC ACIDS
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Crystal structure of an (R)-selective ω-transaminase from Aspergillus terreus (R)-selective ω-transaminase from Aspergillus terre
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Discovery of a novel (R)-selective bacterial cupin HNL from Acidobacterium capsulatum ATCC 51196 by a sequence-based approach
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NR4A1-mediated apoptosis supresses lymphomagenesis and is associated with a favorable cancer specific survival in patients with aggressive B-cell lymphomas
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Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks
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Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastors
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Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris.
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Hunting the needle in the haystack - A guide to obtain biologically meaningful microRNA targets
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Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production
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SEMI-PREPARATIVE SCALE DRUG METABOLITE SYNTHESIS WITH HUMAN FLAVIN MONOOXYGENASESSEMI-PREPARATIVE SCALE DRUG METABOLITE SYNTHESIS WITH HUMAN FLAVIN MONOOXYGENASES135-139
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High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities
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Cofactor Specificity Engineering of Streptococcus mutans NADH Oxidase 2 for NAD (P)+ Regeneration in Biocatalytic Oxidations
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Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation
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Mechanistic study of CMP-Neu5Ac hydrolysis by α2,3-sialyltransferase from Pasteurella dagmatis
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Production of macrocyclic sesqui‐and diterpenes in heterologous microbial hosts: A systems approach to harness Nature’s molecular diversity
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Thermostability improvement of endoglucanase Cel7B from Hypocrea pseudokoningii
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Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16
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Enzymatic decarboxylation—an emerging reaction for chemicals production from renewable resources
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Lipases as Sustainable Biocatalysts for the Sustainable Industrial Production of Fine Chemicals and Cosmetics
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Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression
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Carbon source dependent promoters in yeasts
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PRODUCTION OF RECOMBINANT HUMAN ALDEHYDE OXIDASE IN E. COLI AND OPTIMIZATION OF ITS APPLICATION FOR PREPARATIVE SYNTHESIS OF OXIDIZED DRUG METABOLITES
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Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations
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Microbial Lactone Synthesis Based on Renewable Resources
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MicroRNA-26 family is required for human adipogenesis and drives brown adipocytes characteristics
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Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases.
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Overexpression of membrane proteins from higher eukaryotes in yeasts
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Microbials for the production of monoclonal antibodies and antibody fragments
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Improved selectivity of an engineered multi-product terpene synthase
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Extracellular transaminases for biocatalysisExtracellular transaminases for biocatalysisS198-S198
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Unconventional Enzymatic Reductions: Nitriles and Carboxylates as SubstratesUnconventional Enzymatic Reductions: Nitriles and Carboxylates as SubstratesS1-S2
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Human flavin monooxygenase 2: Heterologous expression in E. coli and API modificationHuman flavin monooxygenase 2: Heterologous expression in E. coli and API modificationS82-S82
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2013
Protein Engineering Using Eukaryotic Expression SystemsProtein Engineering Using Eukaryotic Expression Systems47-72
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Probing the secretory pathway of the yeast Pichia pastorisProbing the secretory pathway of the yeast Pichia pastoris38-38
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Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis
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Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering
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RECENT DEVELOPMENTS IN HYDROXYNITRILE LYASES FOR INDUSTRIAL BIOTECHNOLOGY
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New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris
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Investigation of one-enzyme systems in the w-transaminase-catalyzed synthesis of chiral amines
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First functional expression of cytochrome P450 3A4 in Pichia pastoris
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Regulation of Pichia pastoris promoters and its consequences for protein production
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Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase
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Fusion of binding-domains to Thermobifida cellulosilytica cutinase to tune sorption characteristics and enhancing PET hydrolysis
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Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation
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Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris
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Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants
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Artificial Multi-Enzyme Networks for the Asymmetric Amination of sec-Alcohols
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Enzymatic aerobic alkene cleavage catalyzed by a Mn3+-dependent proteinase A homologue
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A novel cholesterol-producing Pichia pastoris strain is an ideal host for functional expression of human Na,K-ATPase α3β1 isoform
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Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis
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Development of small-molecule inhibitors targeting adipose triglyceride lipase
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YARROWIA LIPOLYTICA DEHYDROGENASE/ REDUCTASE: AN ENZYME TOLERANT FOR LIPOPHILIC COMPOUNDS AND CARBOHYDRATE SUBSTRATES
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Metagenome analyses reveal the influence of the inoculant Lactobacillus buchneri CD034 on the microbial community involved in grass ensiling
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2012
Pichia pastoris as whole-cell biocatalyst for (+)-nootkatone productionPichia pastoris as whole-cell biocatalyst for (+)-nootkatone production48-48
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Recombinant Human Flavin Monooxygenases: Expression and Moclobemide N-oxide Synthesis on Semi-Preparative ScaleRecombinant Human Flavin Monooxygenases: Expression and Moclobemide N-oxide Synthesis on Semi-Preparative ScaleS236-S237
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A NOVEL MULTI-ENZYMATIC ASSAY FOR TRANSAMINASE ACTIVITY: HIGH THROUGHPUT SCREENING APPLICATIONS IN PICHIA PASTORIS
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Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study
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Crystallization of a novel metal-containing cupin from Acidobacterium sp. and preliminary diffraction data analysis
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Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
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Organaktivitäten im Schüttelkolben: Rekombinante menschliche Enzyme für die Pharmaindustrie
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Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage
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Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology
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Expression of lignocellulolytic enzymes in Pichia pastoris
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Sensitive high-throughput screening for the detection of reducing sugars
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Two-step enzymatic functionalisation of polyamide with phenolics
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Recent advances in rational approaches for enzyme engineerings in rational approaches for enzyme engineering
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Characterization of two bacterial hydroxynitrile lyases with high similarity to cupin superfamily proteins
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Molecular characterization of the C-Methyltransferase NovO of Streptomyces spheroides, a valuable enzyme for performing Friedel-Crafts alkylation
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Organ Function in Shake Flasks: Recombinant Human Enzymes for the Pharmaceutical Industry
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Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates
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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway
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The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity
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Characterization of a new cutinase from Thermobifida alba regarding PET-surface hydrolysis
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MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes
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Creation of a lipase highly selective for trans fatty acids by protein engineering
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Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols
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Extracellular serine proteases from Stenotrophomonas maltophilia: screening, isolation and heterologous expression in E.coli.
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High-throughput characterization and comparison of microbial communitiesHigh-throughput characterization and comparison of microbial communities37-57
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Synthesis of Fluorinated Neurotransmitter AnaloguesSynthesis of Fluorinated Neurotransmitter Analogues299-331
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C–X Bond Formation: Hydroxynitrile lyases: From Nature to ApplicationC–X Bond Formation: Hydroxynitrile lyases: From Nature to Application350-371
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A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)
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Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes
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Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale
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NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION
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Crystallisation of the novel S-adenosyl-L-methionine dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun.
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2011
Transcriptional Activation of ZEB1 by Slug Leads to Cooperative Regulation of the EMT like Phenotype in Melanoma
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Hydrolysis of polyethyleneterephthalate by para-nitrobenzylesterase from Bacillus subtilis
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High-quality genome sequence of Pichia pastoris CBS7435
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One-step enzyme extraction and immobilization for biocatalysis applications.
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Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries.
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HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS
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Biocatalytic synthesis of optically active tertiary alcohols
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Biocatalytic strategies for the asymmetric synthesis of profens – recent trends and developments
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Protein engineering of arylmalonate decarboxylase G74C, an artificial racemase
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Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.
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Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining
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Enzymatic Surface Hydrolysis of PET. Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida
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The cadherin switch in melanoma instigated by HGF is mediated through epithelial-mesenchymal transition regulators.
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A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance
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Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones
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Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3
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2010
Microbial PHA Production from Waste Raw MaterialsMicrobial PHA Production from Waste Raw Materials85-119
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Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications
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Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation
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Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases.
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The role of the GGGX motif in determining the activity and enantioselectivity of pig liver esterase towards tertiary alcohols
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Protein engineering and discovery of lipases
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Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents.
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The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering
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Real time PCR based determination of gene copy numbers in Pichia pastoris
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An Enzymatic Toolbox for the Kinetic Resolution of 2-(Pyridin- x -yl)but-3-yn-2-ols and Tertiary Cyanohydrins
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Directed evolution of Alcaligenes faecalis nitrilase
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Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation
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Stepwise engineering of a Pichia pastoris D-amino acid whole cell catalyst
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HGF-promoted motility in primary human melanocytes depends on CD44v6 regulated via NF-kappa B, Egr-1, and C/EBP-beta
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IMPROVED FITNESS OF ARABIDOPSIS THALIANA NITRILASE 2
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C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli.
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Perspectives on synthetic promoters for biocatalysis and biotransformation
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Team Leader
Margit Winkler
Dipl.-Ing. Dr.techn.
